关键词:  , Survivin, 反义寡核苷酸, 原发性肝癌, 转染, 增殖抑制率

Abstract: Abstract:Objective To study the influence of anti-sense oligonucleotides(ASODN) of  Survivin on carcinoma cell proliferation by the technique of ASODN.Methods  Survivin-ASODN was synthesized according to Survivin gene sequence.The SMMC-7721 cells were transfected with fluorescence labled Survivin-ASODN.At the same time,blank control,lipofectamine control and SODN control groups were set up.The Survivin gene expression levels were measured by PCR.The proliferation inhibitory rates of Survivin-ASODN transfected cells were analyzed with MTT method.Results The transfection rate reached 60% of fluorescence labled Survivin-ASODN in SMMC-7721 cells.After transfected with ASODN at 100,200,300,400 and 600 nmol?L-1,the Survivin gene expression levels were decreased.[JP2]The inhibitory rates were (3.87±1.67)%，(9.21±2.21)%，(15.21±3.18)%，(21.32±[JP]3.43)%，and (32.62±3.74)%,respectively,at 24 h after transfection. In 300 nmol?L-1 ASODN group,the inhibitory rates were (29.21±4.12)% at 48 h and (44.21±3.32)% at 72 h.Compared with blank control group,lipofectamine  group and SODN group,the inhibitory rates in ASODN group showed significant difference (P＜0.05).Conclusion Survivin-ASODN has inhibitory effect on cell proliferation in dose-dependent and time-dependent manner.

Key words: Survivin, anti-sense oligonucleotides, liver neoplasms, transfection, inhibitory rate of proliferation