关键词: 髓性, 慢性, 诊断, Ph^1<, sup>染色体, bcr, abl融合基因, 诊断, 鉴别

Abstract: Objective To study the clinical significance of detection of Ph¹ chromosome and bcr/abl fusion gene in the diagnosis for chronic granulocytic leukemia. Methods Ninety-five patients with chronic granulocytic leukemia and twenty patients with iron-deficiency anemia were selected. Bone marrow short-term culture was used to prepare the chromosome, bcr/abl fusion gene was analyzed by RT-PCR method. Results In 95 patients with chronic granulocytic leukemia,83(87.4%) cases were Ph¹ Chromosome positive,90(94.7%)cases were bcr/abl positive,7(7.4%)cases were Ph¹ negative and bcr/abl positive, 5(5.3%) cases were Ph¹ and bcr/abl negative. All 20 patients with iron-deficiency anemia were Ph¹ and bcr/abl negative. Conclusion The results indicated that the detection of Ph¹ chromosome combined with bcr/abl fusion gene could improve the diagnosis and differential diagnosis of chronic granulocytic leukemia.

Key words: myeloid, chronic, diagnosis, Ph^1<, sup> chromosome, bcr, abl fusion gene, diagnosis, differential