关键词: 系统性, 胸腺上皮细胞, 细胞培养, 方法, 小鼠

Abstract: Objective To study and establish the culture system of thymic epithelial cell of BXSB systemic lupus erythematosus mice. Methods The thymic epithelial cells of systemic lupus erythematosus model (BXSB mice) were cultured with merely shearing, shearing and collagenase digestion, shearing and trypsinization in different nutrient media . The cells were identified with microscopy and immunohistochemical methods. Results The pure thymic epithelial cells were obtained not only in the medium with serum but also in the medium with growth factor. The thymic epithelial cells grew very well with shearing and collagenase digestion, and there was seldom contaminated by fibroblast. Passage was operated when the primary culture cells spreaded more than 90% pavement of culture flask. The purity of cells dyed with keratin was more than 95% in the medium with serum by shearing and collagenase digestion. Conclusion The method of shearing and collagenase digestion process is easy and cheap to obtain thymic epithelial cell of BXSB mice. A stable thymic epithelial cell culture system of BXSB systemic lupus erythematosus mice is established.

Key words: systemic, thymic epithelial cells, cell culture, methods, mice