关键词: 诱变剂, 抗诱变药, 人参, 五加, 甘草, 猪苓

Abstract: Objective To study the resistance effect of traditional Chinese medicine mixture to the damage of chromosome caused by Bleomycin (BLM) mutagen. Methods Twenty healthy blood donors were divided into four groups:control group,BLM induced group, traditional Chinese medicine mixture group and traditional Chinese medicine mixture anti-induced group. All blood samples were collected at the day of blood culture, hepalean preventing to coagulate. Culture medium were placed into incubator for 72 h after accepted 0.3 mL blood. The cells were harvested and microscope slides were prepared before Giemsa staining. The influence of BLM on the peripheral blood lymphocyte chromosome of the normal body was detected. The antimutagenic effects of the four-herb mixture including Ginseng，Cortex Acanthopanasia radicis，Liquorice，Grifola umbellate as chromosome stabilizer were observed. Results The b/c value in mutagenesis group (0.733±0.275) was obviously higher than that in control group (0.018±0.076)（P<0.01）, the difference of b/c value between mutagenesis group (0.733±0.275) and traditional Chinese medicine mixture anti-induced group (0.566±0.761) was significant（P<0.01）. Conclusion BLM can increase the fracture rate of the peripheral blood lymphocyte chromosomes in normal body obviously, the mixture including four traditional Chinese medicine can inhibit the fracture rate of chromo some induced by BLM obviously. It suggests that the mixture including four traditional Chinese medicine can improve the genome stability of the organism as chromosome stabilizer.

Key words: mutagens, antimutagenic agents, Panax Ginsen, Acanthopanax Gracilis Tylus, Glycyrrhiza Uralensis, Polyporus Umbellatus