关键词: 壳多糖, 支架, 皮肤, 软骨

Abstract: Objective To explore one simple way of preparation of chitosan extracellular matrix wire frame, and investigate its application as scaffold for tissue engineering. Methods Chitosan and acetic acid were soluted and stirred into uniform foam, and made into porous and spongiform wire frame after refrigeration and casting. The pore size and vacancy volume of the wire frame were determined under light microscope and SEM. Tissue engineered skin and cartilage were reconstructed with chitosan scaffold. Results Chitosan extracellular matrix wire frame looked porous and spongiform, its pores manifested inequality of size under light microscope, and many interlacing pores were found under SEM with pore size of 83-136 μm(average 110 μm). The three diamensions lattice had a vacancy volume of 78%. The tissue engineering skin was similar with natural skin and had bilayer structure. Keratinocytes and fibroblasts sticking on chitosan grew and proliferated, and formed into contiguous cellular layer. They increased obviously and secreted cutin and matrix. The wire frame degraded gradually. Chondrocytes sticking on wire frame grew and proliferated well, and they also secreted extracellular matrix. Generation of new cartilage tissue was found with histological observation. Conclusion The chitosan extracellular matrix wire frame has certain pore size and vacancy volume. And it is fit for cell growth and proliferation. There will be brilliant perspective for application as tissue engineering scaffold.

Key words: chitosan, scaffold, skin, cartilage