关键词: 神经干细胞, 细胞培养, 谷氨酸

Abstract:

Abstract:Objective To study the injury of the neural stem cells cultivated in vitro induced by glutamate of embryonic rats and provide theoretical basis for development of protective drugs of neural stem cells.
Methods The Wistar rat(15 d of gestation) embryonic brain tissues were cultivated  in vitro and the cells were confirmed as the neural stem cells with immunohistochemical method.The neural stem cells cultured  normally were used as control,the different concentrations of glutamate were added,then the survival rate of neural stem cells was observed with MTT colorimetry.Results The differentiation cells from neural stem cells had both neurons and neuroglial cells that presented neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP )dying masculine respectively.After treated with glutamate for 24 h,the survival rates of neural stem cells in glutamate groups were obviously reduced compared with control group.The survival rate in 50 μmol·L^-1  glutamate group was 86.45%,compared with control group,there was no significant difference (P>0.05).The survival rates of the stem cells in 100  and 1 000 μmol·L^-1  glutamate groups (71.22%，15.14%) were significantly lower than that in control group (P<0.05，P<0.01).Conclusion Glutamate has function of damaging on the neural stem cells in a dose-dependent manner;when  the dose of  glutamate  increases,the survival rate of the neural stem cells  decreases gradually.

Key words: neural stem cells, cell culture, glutamate