关键词: 盐霉素, 膀胱肿瘤, 5637细胞, Wnt/&beta, -catenin信号传导途径

Abstract:

Objective To explore the influence of salinomycin in the  growth,apoptosis and invasion of human bladder cancer 5637 cells,and to clarify its possible mechanism.Methods The human bladder cancer 5637 cells cultured in vitro at logarithmic growth phase were divided into control group and different doses of salinomycin(15,30 and 60 μmol?L-1) groups.The inhibitory rate of the growth of 5637 cells in various groups  was measured by MTT assay.Flow cytometry was used to detect the apoptotic rates of 5637 cells in various groups.The invasiveness of 5637 cells was tested by Matrigel Invasion Assay.The  expression levels of β-catenin protein in 5637 cells in various groups were determined by Western blotting method. Results Compared with control group,the inhibitory rates of
 growth of human bladder cancer 5637 cells in different doses of salinomycin groups were increased significantly(P<0.05); the  apoptotic rates were   increased(P<0.05).the number of cells passed the Matrigel was decreased(P<0.05),and the expression level of β-catenin protein was decreased(P<0.05).Compared with low dose of salinomycin group, the inhibitory rate of growth of 5637 cells in high dose of salinomycin group was increased(Ｐ<0.05);the apoptotic rate was increased(P<0.05)，the number of cells passed the Matrigel was decreased(P<0.05),and the expression levels of β-catenin protein was decreased(P<0.05).
Conclusion Salinomycin can inhibit the growth of 5637 cells significantly,increase the apoptosis,and decrease the cell invasion; the inhibitory effect may act by inhibiting the Wnt/β-catenin pathway.

Key words: salinomycin;bladder neoplasms, 5637 cells, Wnt/β-catenin signal transduction pathway