关键词: 五味子乙素, 心肌细胞, 缺氧/复氧损伤, 抗氧化

Abstract:

Abstract:Objective To investigate the influence of  Schizandrain B(Sch B) preconditioning in myocardial cells of neonatal rats with hypoxia / reoxygenation (H/R) injury,and to explore its possible mechanism.Methods The cultured myocardial cells were divided into control group,model group  and 10,50,250 mg/LSchB preconditioning  groups.All the cells in various groups except control group were cultured in 2 mmol/L Na2S2O4  for 2 h，then cultured in normal medium for 18 h to induce myocardial H/R injury.The morphological changes of myocardial cells in various groups were observed under inverted microscope.The survival rates of the myocardial cells in each group were  examined  by MTT.The activities of lactic dehydrogenase(LDH),creatine(CK),superoxide ismutase(SOD),and the (MDA) levels in the cells in various groupswere  examined  by detection kits.Results Compared with control group,the cells in model group were retracted,arrest or float,the survival rate was decreased significantly (P<0.01),the activities of LDH,CK and MDA level were increased (P<0.01),and the SOD activity was decreased (P<0.01);compared with model group,the cells in Sch B preconditioning groups  remained beating,retraction was light,the cell survival rates were significantly increased (P<0.01),the activities of LDH,CK and MDA levels were decreased (P<0.01),and the SOD activities were increased (P<0.05 or P<0.01).Conclusion Sch B has protective effect on  myocardial cells with H/R injury in the neonatal rats,which may be associated with anti-oxidative damage.

Key words: Schisandrin B, myocardial cells, hypoxia/reoxygenation injury, anti-oxygenation