关键词: 疾病模型, 动物, 免疫组织化学

Abstract: Abstract:Objective To explore the feasibility of orthotopic transplantion tumor model of hepatoma in mice and evaluate the biological features.Methods 5×10⁶ H22 cells were inoculated into the liver of mice after anesthetizing.The liver,spleen,lung and kidney were taken from the mice 13 d after inoculation(n=8) or from the died mice(n=9) for morphological evaluation.Proliferating cell nuclear antigen(PCNA) and factor Ⅷ related antigen(FⅧRag) were analyzed with immunohistochemical staining.Results The average survival time of the mice was 18.8 d.Until the 13th day after inoculation,the successful rate of orthotopic transplantation tumor (model of liver cancer) was 100%(8/8),spontaneous lung metastatic rate was 37.5%(3/8).Fifty percent of mice(4/8) produced ascites,and the average amount of ascites was （6.25±3.67）mL.The average size of the tumor was （0.35±0.14） cm³.In the natural died mice,spontaneous lung metastatic rate was 100%（9/9）,100% of mice (9/9)produced ascites. The average volume of acites was （10.88±1.92）mL,which was evidently more than that of the 13th day（P<0.01）.The average size of the tumor was (0.61±0.31) cm³, which was significantly bigger than that of the 13th day（P<0.05）.When the mice died,most of tumor nodules were single and the cellular 〖JP2〗atypia was apparent.The proliferation index and microvessel density of tumor tissue were 71.39%±〖JP〗20.74% and (39.25±13.71)/400 high magnification respectively.Conclusion The successful rate of orthotopic transplantation tumor model of liver cancer is high.The tumor possess high lung metastatic rate and plenty of microvessels and strong proliferative ability.

Key words: disease models, animal , immunohistochemistry