关键词: 癌, 非小细胞肺, 肿瘤坏死因子相关凋亡诱导配体, 细胞凋亡

Abstract: To study the apoptosis induced by tumor necrosis factor related apoptosis-inducing ligand (TRAIL) in non-small cell lung carcinoma H596 cells,and to explore the role and mechanism of apoptosis.Methods Non-small cell lung carcinoma H596 cells were cultivated,and acid phosphate assay was used to detect the apoptotic rate,and the expressions of caspase-8,Bcl-2 and Fas-associated death domain (FADD) proteins were detected by Western blotting in H596 cells.Results  The apoptotic rates of H596 cells were decreased after the cells were treated with 0.01-0.03 μg•L^-1 TRAIL,and from 0.10 μg•L^-1 TRAIL,the apoptotic rates were increased;the differences in apoptotic rates of H596 cells after treated  with 10.00-100.00 μg•L^-1 TRAIL were significant compared with control group(P<0.05).The Western blotting results showed that the expressions of caspase-8,Bcl-2,FADD proteins were normal.Conclusion High dose of TRAIL can induce apoptosis in H596 cells,and it may be  related to the normal expressions of   TRAIL related proteins.

Key words: carcinoma, non-small cell lung, tumor necrosis factor related apoptosis-inducing ligand, apoptosis