浸润性乳腺癌组织中E-cad基因表达和启动子甲基化的检测及其意义

doi:10.13481/j.1671-587x.20140227

吉林大学学报(医学版)

浸润性乳腺癌组织中E-cad基因表达和启动子甲基化的检测及其意义

张一兵，卢鹤翔，张欣然，秦丽娟，董桂兰，孙娜，张田

1.河北联合大学基础医学院生理系，河北唐山 063001； 2. 河北联合大学基础医学院形态实验室，河北唐山 063001；3. 河北省唐山市人民医院放化科，河北唐山 063001

收稿日期:2013-07-15 出版日期:2014-03-28 发布日期:2014-05-28
通讯作者: 张一兵(Tel：0315-3725606，E-mail: dgl01@sina.com) E-mail:dgl01@sina.com
作者简介: 张一兵(Tel：0315-3725606，E-mail: dgl01@sina.com)
基金资助:
国家自然科学基金资助课题(81101912);河北联合大学培育基金资助课题(LDPYS-07)

Detection of E-cad　gene expression and methylation of its promoter in invasive breast cancer tissue and its significance

ZHANG Yi-bing¹,LU He-xiang²,ZHANG Xin-ran,QIN Li-juan¹,DONG Gui-lan³,SUN Na¹,ZHANG Tian¹

1.Department of Physiology,College of Basic Medical Sciences,Hebei United University, Tangshan 063001,China;2.Morphology Laboratory,College of Basic Medical Sciences,Hebei United University,Tangshan 063001,China;3.Department of Radiotherapy and Chemotherapy,People’s Hospital of Tangshan City,Hebei Province,Tangshan 063001,China

Received:2013-07-15 Online:2014-03-28 Published:2014-05-28

摘要/Abstract

摘要：

目的：观察乳腺癌组织中E钙黏素（E-cad）蛋白表达与乳腺癌临床特点、病理学特征以及E-cad基因启动子甲基化的关系，探讨其作为乳腺癌早期诊断和预后评估指标的可行性和临床意义。方法：收集62例乳腺癌组织标本和20例乳腺增生组织标本，采用免疫组织化学法检测E-cad蛋白表达情况；采用甲基化特异性聚合酶链反应（MSP）检测E-cad基因启动子甲基化状态。结果： E-cad蛋白阳性表达以细胞膜为主，乳腺癌组织中E-cad蛋白的阳性表达率（51.6%)低于乳腺增生组织（80.0%）(P<0.05)；乳腺癌组织中E-cad 基因甲基化率（62.9%）高于乳腺增生组织(35.0%) (P<0.05)；乳腺癌组织中，E-cad蛋白表达水平与肿瘤临床分期、组织分化程度和淋巴结转移有关联(P<0.05)；与患者年龄、肿瘤大小、部位及雌激素受体（ER）、孕激素受体（PR）、表皮生长因子受体2（Her-2）的表达无关联（P>0.05）；E-cad蛋白阴性表达的乳腺癌组织中，E-cad基因启动子甲基化率(76.7%)高于E-cad蛋白阳性表达的乳腺癌组织(50.0%)（P<0.05)。结论：启动子甲基化导致的E-cad蛋白表达降低与乳腺癌发生发展有关联，E-cad可能成为乳腺癌患者早期诊断和预后评估的指标。

关键词: , 乳腺肿瘤, E钙黏素, 甲基化, 预后, 启动子

Abstract:

Objective
To observe the relationship between the expression of E-cadherin (E-cad) protein in breast cancer tissue and the clinical characteristics, pathological features and the methylation of E-cad gene promoter of invasive breast cancer,and to investigate the feasibility and clinical significance of E-cad protein as an indictor for early diagnosis and prognosis evaluation of breast cancer. Methods The samples from 62 cases of breast cancer tissue and 20 cases of breast hyperplasia tissue were collected.The expressions of E-cad protein in samples were detected with immunohistochemical assay and the methylation of E-cad gene promoter was detected with methylation-specific polymerase chain reaction (MSP).Results The expression of E-cad protein was mainly in membrane.The positive expression rate of E-cad protein in cancer tissue was lower than that in hyperplasia tissue (51.6% vs 80.0%,P<0.05).The incidence of methylation of E-cad gene promoter in cancer tissue was higher than that in the breast hyperplasia tissue (62.9% vs 35.0%,P<0.05).The expression level of E-cad protein was correlated with TNM stage,histological differentiation degree and lymph node metastasis (P<0.05).There was no correlation between the patient’s age,tumor size,lesion location and the expressions of estrogen receptor(ER),progesterone receptor(PR),and epidermal growth factor receptor(Her-2) (P>0.05).The methylation rate of E-cad gene promoter in E-cad protein negative expression tissue was higher than that in E-cad protein positive expression tissue(P<0.05).Conclusion The decrease of E-cad protein expression caused by E-cad gene promoter methylation is involved in the occurrence and development of breast cancer.E-cad may be one of the predictors for early diagnosis and prognosis evaluation of breast cancer.

Key words: breast neoplasms, E-cadherin, methylation, prognosis, promoter

中图分类号:

R737.9

张一兵，卢鹤翔，张欣然，秦丽娟,董桂兰，孙娜，张田. 浸润性乳腺癌组织中E-cad基因表达和启动子甲基化的检测及其意义[J]. 吉林大学学报(医学版), 2014, 40(02): 358-362.

ZHANG Yi-bing,LU He-xiang,ZHANG Xin-ran,QIN Li-juan,DONG Gui-lan,SUN Na,ZHANG Tian . Detection of E-cad　gene expression and methylation of its promoter in invasive breast cancer tissue and its significance[J]. Journal of Jilin University Medicine Edition, 2014, 40(02): 358-362.

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浸润性乳腺癌组织中E-cad基因表达和启动子甲基化的检测及其意义

Detection of E-cad　gene expression and methylation of its promoter in invasive breast cancer tissue and its significance

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浸润性乳腺癌组织中E-cad基因表达和启动子甲基化的检测及其意义

Detection of E-cad gene expression and methylation of its promoter in invasive breast cancer tissue and its significance

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Detection of E-cad　gene expression and methylation of its promoter in invasive breast cancer tissue and its significance