关键词: 北五味子提取物, 肝纤维化, 白蛋白, 自由基, 大鼠, Wistar

Abstract:

Abstract:Objective
To study the protective effects of extract fructus Schisandrae Chinensis (EFSC) on hepatic injury in experimental hepatic fibrosis rats,and to clarify its mechanism.Methods 60  Wistar rats were randomly divided into control group(n=10),model group(n=10),malotilate group(n=10),and 100,200 and 400 mg/kg EFSC groups(n=10).The experimental hepatic fibrosis model was established by hypodermic injection with CCl4  arachis oil solution for 10 weeks.The activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT),and the levels of albumin (ALB),globulin (GLO),total protein (TP) and nitrogen monoxidum (NO) in serum of the rats in various groups were determined;the activities of superoxide dismutase (SOD) and nitricoxide synthase (NOS),and the levels of malonaldehyde (MDA),glutathion (GSH) and hydroxyproline (Hyp) in liver tissue of the rats in various groups were detected.Results Compared with model group,after treated with  200 and 400 mg/kg  EFSC  for 4 weeks,the levels of ALB and ALB/GLO ratio in serum of the rats were significantly increased (P<0.05 or P<0.01);the activities of AST and ALT and the  levels of GLO and NO in serum of the rats were significantly decreased (P<0.05 or P<0.01);the activities of SOD and the levels of GSH in liver  tissue of the rats were   significantly increased(P<0.05 or P<0.01) and the levels of MDA and Hyp in liver tissue of the rats were significantly decreased(P<0.05).Conclusion EFSC can improve the hepatic function,inhibit the generation of hepatic collagen fiber,correct the low level of ALB and lighten the damage of lipid peroxidation in hepatocytes,so as to prevent formation and development of hepatic fibrosis.

Key words: extract fructus Schisandrae Chinensis；hepatic fibrosis, albumin, free radical, rats,Wistar