One SD rat was selected， the chest was opened， and the aorta was removed in sterile condition； the connective tissue and fat outside the blood vessel were removed；the aortic tunica intima was everted and the aorta were cut into 1.0－1.5 mm vascular segments，and the vascular segments were placed in a culture bottle for primary culture. Then the vascular segments were removed 7 d later. The cultured cells were identified by morphological observation and immunocytochemical staining of factor Ⅷ（FⅧ） related antigen.

After 3 d of culture， a small number of cells migrated from the vascular segments to form the island cell mass. From the 10th to 12th days， the adherent cells covered most of the culture bottle surface， and presented a typical “paving stone” mosaic arrangement. The results of immunocytochemical staining of FⅧ showed that the nucleus and the cytoplasm were brownish red，and the expression was positive； the positive cell rate was more than 98%.

Adherent culture method of aortic intima valgus can successfully isolate and culture the primary rat AECs.