Forty kunming mice of clean grade were used to establish fatigue animal models by the weight-bearing swimming test. They were randomly divided into control group， low， medium and high doses （0.25， 0.50 and 1.00 g·kg^－1） of GARP groups， and there were 10 mice in each group. The swimming time of the mice in various groups was recorded through the exhaustive swimming experiment. The mice were killed after swimming experiment. The spectrophotometric method was used to detect the blood lactic acid （BLA） and blood urea nitrogen （BUN） levels in the serum of the mice in various groups. The spectrophometric method was used to detect the glutathione （GSH） levels， the superoxide dismutase （SOD） activities and the liver glycogen levels in liver tissue of the mice in various groups， and the spectrophometric method was used to detect the muscle glycogen level in the nuscle tissue of the mice in various groups. The C2C12 myoblasts were divided into control group and different doses （5， 10 and 20 mg·L^－1） of GARP groups.The spectrophotometric method was used to detect the GSH levels， SOD activities， and the glycogen levels in the cells in various groups， and the glucose uptakes of the cells in various groups were determined with phenol sulphuric acid process.The phosphorylation levels of adenosine 5'-monophosphate （AMP）-activated protein kinase （AMPK） （p-AMPK/AMPK） and the expression level of glucose transporter 4 （GLUT4） protein were detected by Western blotting method.

Compared with control group， the swimming time of mice in medium and high doses of GARP groups was increased （P<0.05 or P<0.01）； the BLA and BUN levels in the serum of the mice were reduced （P<0.05 or P<0.01）， the GSH levels and SOD activities in different doses of GAPP groups were increased （P<0.05 or P<0.01）； the liver glycogen and muscle glycogen levels were increased （P<0.05 or P<0.01）. Compared with control group the GSH levels，the SOD activities and the glycogen levels in the C2C12 myoblasts in 5，10 and 20 μg·mL^－1 GARP groups were increased （P<0.05 or P<0.01）， the glucose uptakes were increased （P<0.05 or P<0.01），and the ratios of p-AMPK AMPK and the expression levels of GLUT4 protein were increased （P<0.05 or P<0.01）.

GARP has anti-fatigue effect，and its mechanism may be achieved by activating AMPK/GLUT4 signaling pathway to promote glucose uptake.