The Comparative Toxicogenomics（CTD） and GeneCards databases were used to predict the intersection mRNA related to rutin； Gene Ontdogy（GO） and Kyoto Encyclopedia of Genes and Genones（KEGG） databases were used to predict the biological function and action pathways of the intersection mRNA； String database was used to find the Notch-1 related proteins. The human CRC SW480 cells were used as the subjects， 0.1， 1.0， and 10.0 μmol·L^－1 DAPT were given for preliminary screening， then 1， 2， 4， 8 and 10 μmol·L^－1 DAPT were given to intervene， and the optimal concentrations of rutin and DAPT were screened out. The human CRC SW480 cells were divided into blank control group， rutin group （22 μmol·L^－1）， DAPT group （1 μmol·L^－1） and rutin+DAPT group（22 μmol·L^－1 rutin+1 μmol·L^－1 DAPT）.MTT method was used to detect the cell proliferation activities； Hoechst33258 nuclear staining was used to observe the apopotic morphology of cells，and flow cytometry was used to detect the apoptotic rates；real-time fluorescence quantitative PCR（RT-qPCR） method was performed to detect the expression levels of Notch-1 mRNA in the SW480 cells in various groups； Western blotting method was used to determine the protein expression levels of Notch-1，caspase-3，caspase-9，B-cell lymphoma 2（Bcl-2），and Bcl-2 associated X protein in the SW480 cells in various groups.

In CTD and GeneCards databases， there were a total of 34 intersection mRNA；the GO and KEGG databases predicted that the intersection mRNA was related to the process of colon cancer apoptosis； String database verified 16 Notch-1 related proteins （score>0.42）； the optimal administration concentrations of rutin and DAPT were 22 μmol·L^－1 and 1 μmol·L^－1； compared with blank control group， the apoptotic rates in rutin group， DAPT group and rutin+DAPT group were increased significantly （P<0.05）， the expression levels of Notch1 mRNA and protein were significantly reduced （P<0.05）， the expression levels of caspase-3， caspase-9， and Bax proteins in the cells were increased significantly（P<0.05）， while the expression levels of Bcl-2 protein were decreased significantly （P<0.05）. There was no significant difference in the apoptotic rate between rutin group and DAPT group （P>0.05）. Compared with rutin group and DAPT group， the apoptotic rate in rutin+DAPT group was significantly increased（P<0.05）， the expression levels of caspase-3， caspase-9， and Bax proteins were increased significantly（P<0.05），while the expression level of Bcl-2 protein was decreased significantly（P<0.05）.

The results of bioinformatics analysis and experiment show that rutin can promote the apoptosis of CRC cells， which may be achieved by targeting Notch-1 and regulating MAPK signal pathway.