Abstract:

Objective To investigate the carbapenem drug resistance of the intensive care unit（ICU） patients infected with Acinetobacter baumannii（AB）， and to clarify the clinical application value of real-time fluorescence quantitative PCR（RT-qPCR） method for the detection of drug resistance genes. Methods A total of 285 sputum specimens from the ICU patients were collected for identification of the drug susceptibility by traditional culture method and detection of the drug resistance genes by RT-qPCR method. Statistical analysis was performed on the detection rate of the carbapenem-resistant Acinetobacter baumannii （CRAB）， the coincidence rate of the drug resistance gene detection and the drug resistance of other antibiotics. Results A total of 151 strains of AB were obtained， the detection rate was 52.98%. The drug resistance rate of AB to carbapenem was 72.20%.There was no significant difference in the coincidence rate of OXA-51 gene detected by RT-qPCR method compared with the traditional culture method（P>0.05）. There was no significant difference in the coincidence rate of CRAB detected by OXA-23 gene compared with the traditional culture method （P>0.05）.The drug resistance rates of 108 OXA-23 positive specimens infected with AB to minocycline，cefoperazone/sulbactam， myxin，and tegacyclin were lower. Conclusion The detection rate of carbapenem resistant drugs of AB identified by sputum culture of the ICU patients is high. Compared with traditional culture method， RT-qPCR method is simpler， faster and has a higher consistent rate of detection.The drug resistance rates of CRAB to minocycline， cefoperazone/sulbactam， myxin and tegacyclin are low.

Key words: Carbapenem-resistant Acinetobacter baumannii, Intensive care unit, Drug resistance gene, Real-time fluorescence quantitative PCR