Abstract:

Objective To screen the active ingredients of traditional Chinese medicine Sanghuang in the treatment of pneumonia based on network pharmacology， and to analyze the active ingredients and targets of Sanghuang in the treatment of pneumonia. Methods The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform （TCMSP） software was used to search for the ingredients and targets of Sanghuang； the Gene Name was obtained from the Uniprot database； the targets corresponding to pneumonia were found through GeneCards， and the targets of Sanghuang and pneumonia were compared to obtain the key targets； Cytoscape 3.9.1 was used to draw the chemical ingredients of Sanghuang-pneumonia-target network diagram； the protein-protein interaction （PPI） network diagram was constructed by String platform； Gene Ontology （GO） biological function analysis and Kyoto Encyclopedia of Genes and Genomes（KEGG） signaling pathway enrichment analysis on key genes of Sanghuang were performed by using the ClueGo plugin in Cytoscape software. Results There were 21 kinds of active ingredients in the traditional Chinese medicine Sanghuang， and （E）-4-（3，4-dihydroxyphenyl）but-3-en-2-one contained the most targets， including cytochrome P450 family （CYP450）， human epidermal growth factor receptor （EGFR）， and matrix metalloproteinase （MMP） and so on. A total of 358 targets were screened as the compound targets of Sanghuang，among them 217 genes were regarded as common targets for Sanghuang and pneumonia， and 69 key genes related to pneumonia were amine oxidase copper containing 3 gene （AOC3）， DNA ligase 1 gene （LIG1）， glutamyl aminopeptidase gene （ENPEP）， aldehyde dehydrogenase 2 family member gene （ALDH2）， PHD finger protein 8 gene （PHF8）， solute carrier family 11 member 2 gene （SLC11A2），CYP50 and so on.The KEGG and GO analysis results showed that Sanghuang mainly produced a marked effect through 17 metabolic pathways， including the phosphatidylinositol-3 kinase/protein kinase B （PI3K/Akt） signaling pathway， microRNAs（miRNA） in cancer， chemical carcinogenesis - receptor activation， prostate cancer， proteoglycans and lipids in cancer， atherosclerosis， chemical carcinogenesis-reactive oxygen species （ROS） and other related pathways. Conclusion （E）-4-（3，4-dihydroxyphenyl）but-3-en-2-one is the most effective chemical substance in the treatment of pneumonia among the ingredients of Sanghuang， and its mechanism is mainly related to the CYP450 family.

Key words: Sanghuang, Pneumonia, Network pharmacology, Signaling pathway, Target