红景天苷对高原红细胞增多症模型大鼠骨髓CD71+有核红细胞凋亡的影响

doi:10.13481/j.1671-587X.20230510

Abstract

Abstract:

Objective To discuss the effect of salidroside on the apoptosis of the bone marrow CD71+ nucleated red blood cells（RBC） in the rat model of high altitude polycythemia （HAPC），and to clarify its mechanism. Methods orty-five male SD rats were randomly divided into control group （exposed to 1 500 m altitude + NaCl solution）， model group （exposed to 5 000 m altitude + NaCl solution）， and drug group （exposed to 5 000 m altitude + 0.15 g·kg^－1·d^－1 salidroside），and there were 15 rats in each group. The RBC counts， hemoglobin （Hb） levels， and hematocrit （HCT） levels of the rats in various groups were detected； flow cytometry was used to detect the percentages of bone marrow CD71+nucleated RBC， apoptotic rates of bone marrow CD71+nucleated RBC， levels of mitochondrial membrane potential （MMP） and expression levels of cysteinyl aspartate-specific protease-3 （Caspase-3） in bone marrow CD71+nucleated RBC of the rats in various groups； Western blotting method was used to detect the expression levels of B-cell lymphoma-2 （Bcl-2）， Bcl2-associated X protein （Bax），and cysteinyl aspartate-specific protease-9 （Caspase-9） proteins in bone marrow CD71+nucleated RBC of the rats in various groups. Results Compared with control group， the RBC count， Hb level， HCT level of the rats in model group were increased （P<0.01）， and the percentage and apoptotic rate of bone marrow CD71+nucleated RBC were increased （P<0.01）， and the expression levels of Bax， Caspase-9， and Caspase-3 proteins in the bone marrow CD71+nucleated RBC were increased （P<0.05）. Compared with model group， the percentage of bone marrow CD71+nucleated RBC of the rats in drug group was decreased （P<0.01）， the apoptotic rate of bone marrow CD71+nucleated RBC（P<0.01）， and the MMP level in the bone marrow CD71+nucleated RBC were increased （P<0.05）， and the expression levels of Caspase-3， Bax， and Caspase-9 proteins in the bone marrow CD71+nucleated RBC were decreased （P<0.01）. Conclusion Salidroside can effectively inhibit the increasing of apoptosis of nucleated RBC in bone marrow of the HAPC rats and improve the excessive accumulation of the RBC caused by hypoxia， which may have a certain preventive and protective effect in the pathogenesis of HAPC in the rats.

Key words: High altitude polycythemia, Salidroside, Bone marrow mononuclear cells, Apoptosis, Red blood cells

CLC Number:

R551.3

Yong GUO,Shengyan WANG,Jingjing YI,Sen CUI. Effect of salidroside on apoptosis of CD71+ nucleated red blood cells in bone marrow in high altitude polycythemia model rats[J].Journal of Jilin University(Medicine Edition), 2023, 49(5): 1174-1181.

Figures/Tables 5

Tab. 1

Tab. 2

Fig. 1

Tab. 3

Fig. 2

References 27

1	国际高原医学会慢性高原病专家小组. 第六届国际高原医学和低氧生理学术大会颁布慢性高原病青海诊断标准［J］. 青海医学院学报， 2005， 26（1）： 3-5.
2	中华中医药学会. 高原红细胞增多症中医临床诊疗指南［J］. 中国高原医学与生物学杂志， 2021， 42（3）： 205-216.
3	BAI X L， DENG X L， WU G J， et al. Rhodiola and salidroside in the treatment of metabolic disorders［J］. Mini Rev Med Chem， 2019， 19（19）： 1611-1626.
4	贾守宁，陈文娟，李欣，等. 红景天苷对高原红细胞增多症大鼠促红细胞生成素及骨髓病理学影响的研究［J］. 上海中医药杂志， 2020， 54（7）： 94-98.
5	贾守宁，孙梦茹，马春花，等. 红景天苷对高原红细胞增多症大鼠红细胞膜结构和功能的影响［J］. 中草药， 2020， 51（15）： 3960-3967.
6	李晓栩，黄瑊，杨诚忠，等. 复制慢性高原病动物模型实验教学中注意事项分析［J］. 现代医药卫生， 2015， 31（9）： 1410-1412.
7	格桑罗布，达娃次仁，古桑拉姆，等. 一种新的高原红细胞增多症大鼠模型的建立［J］. 西藏医药， 2019， 40（5）： 25-29.
8	董旭. 便携式膜法氧气机在模拟高原慢性缺氧环境下对大鼠血液指标的影响［J］.高原医学杂志，2018，28（1）： 32.
9	DENG B N， LIU W L， PU L L， et al. Quantitative proteomics reveals the effects of resveratrol on high-altitude polycythemia treatment［J］. Proteomics， 2020， 20（14）： e1900423.
10	MA J， JI L H， LI Z Q， et al. Downregulation of intrinsic apoptosis pathway in erythroblasts contributes to excessive erythrocytosis of chronic mountain sickness［J］. Blood Cells Mol Dis， 2019， 76： 25-31.
11	廖瑜，王玮，冯琳，等. 慢性低氧对小鼠骨髓有核红细胞分化与凋亡的调控作用［J］. 中国高原医学与生物学杂志， 2018， 39（1）： 19-23.
12	格桑罗布，古桑拉姆，达娃次仁，等. 高原红细胞增多症模型大鼠心脏病理特征分析［J］.西藏医药，2020，41（1）： 16-19.
13	格桑罗布，古桑拉姆，达娃次仁，等. 高原红细胞增多症模型大鼠肺组织病理特征分析［J］. 西藏医药， 2020， 41（2）： 24-27.
14	WANG S Y， CUI S， LI Z Q， et al. Study on apoptosis， cytochrome C and mitochondrial membrane potential in CD71（+） nucleated erythrocytes in patients with chronic mountain sickness［J］. Chin Med J， 2018， 98（7）： 514-518.
15	刘晴，赵敏，刘丽娜，等. 地西他滨联合硼替佐米和地塞米松治疗复发或难治性多发性骨髓瘤的疗效分析［J］.中国实用内科杂志，2022，42（5）：414-417.
16	SHAKERI R， KHEIROLLAHI A， DAVOODI J. Apaf-1： regulation and function in cell death［J］. Biochimie， 2017， 135： 111-125.
17	BOGNER C， KALE J， POGMORE J， et al. Allosteric regulation of BH3 proteins in bcl-x1 complexes enables switch-like activation of bax［J］. Mol Cell， 2020，77（4）： 901-912.e9.
18	LAN M， KONG Z D， LIU F J， et al. Activating Caspase-8/Bid/ROS signaling to promote apoptosis of breast cancer cells by folate-modified albumin baicalin-loaded nanoparticles［J］.Nanotechnology，2022，33（43）：353-359.
19	CHI X K， NGUYEN D， PEMBERTON J M， et al. The carboxyl-terminal sequence of BIM enables bax activation and killing of unprimed cells［J］. Elife， 2020， 9： e44525.
20	邓戈，贾守宁，李军茹，等. 红景天苷对高原红细胞增多症大鼠防治作用的实验研究［J］. 新中医，2016，48（5）： 304-306.
21	贾守宁，李军茹，马春花，等. 红景天苷对高原红细胞增多症大鼠耳廓微循环的影响［J］. 云南中医中药杂志， 2016， 37（2）： 56-57.
22	GUO C C， LI Y， ZHANG R， et al. Protective effect of salidroside against diabetic kidney disease through inhibiting BIM-mediated apoptosis of proximal renal tubular cells in rats［J］. Front Pharmacol， 2018， 9： 1433.
23	YAN R， XU H， FU X X. Salidroside protects hypoxia-induced injury by up-regulation of miR-210 in rat neural stem cells［J］. Biomedecine Pharmacother， 2018， 103： 1490-1497.
24	XUE X H， FENG Z H， LI Z X， et al. Salidroside inhibits steroid-induced avascular necrosis of the femoral head via the PI3K/Akt signaling pathway： in vitro and in vivo studies［J］. Mol Med Rep， 2018， 17（3）： 3751-3757.
25	邬玫竹，项云，鲍翠玉，等. 基于AMPK/mTOR/p70S6K通路探讨红景天苷对高脂所致H9c2心肌细胞凋亡的保护作用［J］. 中国中药杂志， 2022， 47（14）： 3837-3843.
26	张常银，陶众杰，朱晨杰. 红景天苷对不育症大鼠睾丸生精细胞增殖凋亡的影响［J］. 中华全科医学， 2022， 20（5）： 761-765.
27	魏玉萍，白海，孙延庆，等. 红景天苷对阿糖胞苷诱导的骨髓间充质干细胞凋亡的影响［J］. 中国实验血液学杂志， 2013， 21（6）： 1572-1577.

Group	Count of RBC（×10¹² L^－1）	Level of Hb[ρ_B/（g·L^－1）]	Level of HCT（η/%）
Control	5.24±0.37	131.93±10.19	35.99±2.71
Model	8.79±0.52^*	218.33±17.49^*	59.53±5.31^*
Drug	7.22±0.60^△	190.73±10.73^△	52.59±3.66^△
F	187.08	166.98	134.50
P	<0.001	<0.001	<0.001

Group	Pecentage of nucleated RBC （η/%）	Apoptotic rate（η/%）	MMP（η/%）	Caspase-3 protein
Control	15.98±3.58	28.50±5.00	82.01±2.78	32.91±1.38
Model	27.12±2.64^*	40.65±5.15^*	80.32±4.44	50.52±2.28^*
Drug	22.73±1.06^△	1.17±0.55^△△	91.24±10.95^△	3.28±2.13^△△
F	28.10	7.46	7.39	830.58
P	<0.001	<0.001	0.20	<0.001

Group	Bcl-2 protein	Bax protein	Caspase-9 protein
Control	0.93±0.37	0.59±0.11	1.45±0.38
Model	0.92±0.36	1.05±0.53^*	1.85±0.20^*
Drug	0.53±0.27	0.32±0.17^△	0.94±0.47^△
F	3.27	10.109	12.696
P	0.06	0.01	<0.001

[1]	Yang LI,Shiyang LI,Hongmei ZHANG,Qianqian LIU,Yubing CHEN. Effect of NGFR gene expression differences on promotion of apoptosis of squamous cell carcinoma and adenocarcinoma cells by PD-1 inhibitor-activated T lymphocytes [J]. Journal of Jilin University(Medicine Edition), 2026, 52(3): 680-693.
[2]	Qiaotong REN,Weihua WU,Xingxiang WANG,Shichao WANG,Yipeng CHENG,Chun LI. Effect of interleukin-17 recepter B gene silencing on proliferation, invasion and apoptosis of lung adenocarcinoma A549 cells and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2026, 52(3): 738-745.
[3]	Qiuping SU,Lei XING,Wei ZHANG,Shan JIANG,Yangyang ZHAO,Fangfang CHEN. Research progress in anti-tumor mechanism of berberine and application of its novel nano-delivery system in tumor therapy [J]. Journal of Jilin University(Medicine Edition), 2026, 52(3): 863-871.
[4]	Jie YU,Zongkang WANG,Xun LIU,Yanan YANG,Jin TAN. Effect of miR-153-3p/PGC-1α axis on apoptosis of oral squamous cell carcinoma cells by modulation of mitochondrial apoptosis pathway [J]. Journal of Jilin University(Medicine Edition), 2026, 52(2): 348-358.
[5]	Yaping LI,Chunyan TAN,Lujie ZHAO,Jiayi ZHAO,Ting LI,Xiao YANG,Xiaoyun YANG. Inductive effect of lysophosphatidic acid combined with 6-hydroxydopamine on apoptosis of SH-SY5Y cells and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2026, 52(1): 152-161.
[6]	Jiaxin WANG,Junwen MAO,Xuan ZHANG. Expression of natural autoantibodies against apoptosis inhibitory genes in plasma of patients with hepatocellular carcinoma and its clinical significance [J]. Journal of Jilin University(Medicine Edition), 2026, 52(1): 228-235.
[7]	Huaimin LIANG,Jiacheng JIN,Wenhua CHEN,Yuyao LI,Hangyu WANG,Ke ZHANG,Jinhui WANG. UPLC-Q-TOF/MS and network pharmacology analysis and experimental verification based on potential active ingredients and mechanisms of medicinal Mulberry Leaves in anti-acute kidney injury [J]. Journal of Jilin University(Medicine Edition), 2026, 52(1): 56-69.
[8]	Shanshan SUN,Mei LU,Xinfu GAO,LYu Guangyao,Baolei Zhao,LYu Wenwen. Inhibitory effect of Bradykinin 1 receptor antagonist ELN441958 on proliferation of HepG2 cells by regulating Akt/FoxO3a signaling pathway [J]. Journal of Jilin University(Medicine Edition), 2026, 52(1): 70-80.
[9]	Xiaohan YAO,Mingchen YAO,Zhiqing WANG,Heyang LI,Yan YAN,Ningjing LEI. Effect of silencing GPR139 gene on proliferation, apoptosis and autophagy of breast cancer cells and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2026, 52(1): 1-9.
[10]	Han XUE,Yuxin FAN,Ting ZHANG,Zhimin LI,Mingge HUO,Xingang GUAN. Preparation of nanodrug PTX₂ NPs and its killing effect on human lung cancer A549 cells [J]. Journal of Jilin University(Medicine Edition), 2025, 51(5): 1260-1266.
[11]	Jiarui LI,Zhenlin YANG,Fan GAO,Jingjing GUO,Jinzi LI. Effect of miR-34a-5p on hippocampal neuron apoptosis in rats with temporal lobe epilepsy and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2025, 51(4): 939-947.
[12]	Panxi SUN,Xue QIN,Chongyang ZHANG,Jia LUO,Yong CHEN,Lili WEI. Protective effect of TUG-891 on ischemic stroke induced by ischemia and hypoxia and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2025, 51(4): 968-975.
[13]	Cheng CHEN,Jingyao LI,Wanxiang HU,Donghui LIU,Zhihong CHEN. Protective effect of sericin on streptozotocin-induced INS-1 cell damage by regulating PI3K/Akt/NF-κB signaling pathway through Akt1 and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2025, 51(3): 590-598.
[14]	Donghui LIU,Yunzhe CI,Chunyan WANG,Wenyi MA. Effect of miR-199a-5p on expression of Caveolin-1, cell migration and apoptosis in glioma U251 cells [J]. Journal of Jilin University(Medicine Edition), 2025, 51(3): 663-671.
[15]	Shuyan SUN,Huakun ZHANG,Ziru ZHOU,Feng LI,Xiaobin CUI. Expression of CRNN protein in esophageal squamous cell carcinoma tissue and influence of its overexpression in biological behavior of esophageal squamous cell carcinoma Eca9706 cells [J]. Journal of Jilin University(Medicine Edition), 2025, 51(2): 275-283.

Effect of salidroside on apoptosis of CD71+ nucleated red blood cells in bone marrow in high altitude polycythemia model rats

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 27

Related Articles 15

Metrics

Comments

Recommended 10