TRIM24蛋白在人肾透明细胞癌组织中的表达及其临床意义

doi:10.13481/j.1671-587X.20250223

Abstract

Abstract:

Objective To discuss the expression of tripartite motif-containing protein 24 （TRIM24） in the clear cell renal cell carcinoma （ccRCC） tissue， and to clarify its relationships with the clinicopathological features and prognosis of the ccRCC patients. Methods The cancer and paracancer normal tissues were collected from 90 ccRCC patients who had not undergone preoperative radiotherapy or chemotherapy. Tissue microarray and immunohistochemistry were used to detect the expression levels of TRIM24 protein in ccRCC tissue. The differences in TRIM24 protein expression between ccRCC and paracancer normal tissues were analyzed. The score of TRIM24 protein expression and the average value were calculated， and based on the average value， the patients were classified into TRIM24 protein low-expression and TRIM24 protein high-expression groups. The associations between the TRIM24 protein expression and different clinicopathological features of the patients were analyzed， and the relationship between the TRIM24 protein expression and the prognosis of the patients was analyzed. Results The immunohistochemistry results showed that the TRIM24 protein was expressed in both the nucleus and cytoplasm of the ccRCC tumor cells， and there were significant differences in the TRIM24 protein expression level in ccRCC tissue when compared with paracancer normal tissue （P<0.05）. The TRIM24 protein expression in the nucleus of ccRCC tissue was associated with the patient’s age， gender， and tumor size （P<0.05）. The Kaplan-Meier survival analysis results showed that the overall survivals of the patients with high TRIM24 protein expression in the cytoplasm of ccRCC tissue， older age， and higher pathological grade were shorter than those with low TRIM24 protein expression， younger age， and lower pathological grade （P<0.05）. The multivariate Cox regression analysis results showed that the prognosis of the patients with high TRIM24 protein expression in the cytoplasm and higher pathological grade were poorer compared with the patients with low TRIM24 protein expression and lower pathological grade （P<0.05）. Conclusion The ccRCC patients with high TRIM24 expression in the cytoplasm of ccRCC tumor tissue and higher pathological grade have the lower postoperative survival rates and poorer prognosis.

Key words: Clear cell renal cell carcinoma, Triple motif protein 24, Immunohistochemistry, Pathological grade, Prognosis

CLC Number:

R737.11

Haiying GENG,Yan YU,Chunmei DAI,Youfeng WEN,Ning LI. Expression of TRIM24 protein in human clear cell renal cell carcinoma tissue and its clinical significance[J].Journal of Jilin University(Medicine Edition), 2025, 51(2): 486-492.

Figures/Tables 5

Fig. 1

Fig. 2

Tab. 1

Fig. 3

Tab.2

References 25

1	PADALA S A， BARSOUK A， THANDRA K C， et al. Epidemiology of renal cell carcinoma［J］. World J Oncol， 2020， 11（3）： 79-87.
2	LJUNGBERG B， ALBIGES L， ABU-GHANEM Y， et al. European association of urology guidelines on renal cell carcinoma： the 2022 update［J］. Eur Urol， 2022， 82（4）： 399-410.
3	ROSELLINI M， MARCHETTI A， MOLLICA V， et al. Prognostic and predictive biomarkers for immunotherapy in advanced renal cell carcinoma［J］. Nat Rev Urol， 2023， 20（3）： 133-157.
4	PETITPREZ F， AYADI M， DE REYNIÈS A， et al. Review of prognostic expression markers for clear cell renal cell carcinoma［J］. Front Oncol， 2021， 11： 643065.
5	梅英. USP4促进肝细胞癌增殖和转移及其机制研究［D］. 重庆：重庆医科大学， 2018.
6	YAO Y B， ZHOU S， YAN Y， et al. The tripartite motif-containing 24 is a multifunctional player in human cancer［J］. Cell Biosci， 2024， 14（1）： 103.
7	LE DOUARIN B， NIELSEN A L， YOU J， et al. TIF1 alpha： a chromatin-specific mediator for the ligand-dependent activation function AF-2 of nuclear receptors？［J］. Biochem Soc Trans， 1997， 25（2）： 605-612.
8	LE DOUARIN B， NIELSEN A L， GARNIER J M， et al. A possible involvement of TIF1 alpha and TIF1 beta in the epigenetic control of transcription by nuclear receptors［J］. EMBO J， 1996， 15（23）： 6701-6715.
9	LIU J， ZHANG C， WANG X， et al. Tumor suppressor p53 cross-talks with TRIM family proteins［J］. Genes Dis， 2020， 8（4）： 463-474.
10	STONE L. Prostate cancer： TRIM24 acts as a transcriptional activator［J］. Nat Rev Urol， 2016， 13（8）： 437.
11	TSAI W W， WANG Z X， YIU T T， et al. TRIM24 links a noncanonical histone signature to breast cancer［J］. Nature， 2010， 468（7326）： 927-932.
12	ZHANG L H， YIN Y H， CHEN H Z， et al. TRIM24 promotes stemness and invasiveness of glioblastoma cells via activating Sox2 expression［J］. Neuro Oncol， 2020， 22（12）： 1797-1808.
13	ZHANG L W， CHEN H， DING B J， et al. High expression of TRIM24 predicts worse prognosis and promotes proliferation and metastasis of epithelial ovarian cancer［J］. J Ovarian Res， 2022， 15（1）： 19.
14	YU Y P， CAI L C， WANG X Y， et al. BMP8A promotes survival and drug resistance via Nrf2/TRIM24 signaling pathway in clear cell renal cell carcinoma［J］. Cancer Sci， 2020， 111（5）： 1555-1566.
15	CUI Z B， CAO W， LI J， et al. TRIM24 overexpression is common in locally advanced head and neck squamous cell carcinoma and correlates with aggressive malignant phenotypes［J］. PLoS One， 2013， 8（5）： e63887.
16	TIAN H， ZHAO H M， QU B， et al. TRIM24 promotes colorectal cancer cell progression via the Wnt/β-catenin signaling pathway activation［J］. Am J Transl Res， 2022， 14（2）： 831-848.
17	XUE D W， ZHANG X W， ZHANG X L， et al. Clinical significance and biological roles of TRIM24 in human bladder carcinoma［J］. Tumour Biol， 2015， 36（9）： 6849-6855.
18	JIANG T， MAO H P， CHEN Q， et al. Trim24 prompts tumor progression via inducing EMT in renal cell carcinoma［J］. Open Med （Wars）， 2020， 15（1）： 1153-1162.
19	VENUTO S， MERLA G. E3 ubiquitin ligase TRIM proteins， cell cycle and mitosis［J］. Cells， 2019， 8（5）： 510.
20	WANG Y， YAO Y B， WEI Q H， et al. TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex［J］. Oncogene， 2023， 42（8）： 586-600.
21	HUANG N， SUN X L， LI P， et al. TRIM family contribute to tumorigenesis， cancer development， and drug resistance［J］. Exp Hematol Oncol， 2022， 11（1）： 75.
22	KHETCHOUMIAN K， TELETIN M， TISSERAND J， et al. Loss of Trim24 （Tif1alpha） gene function confers oncogenic activity to retinoic acid receptor alpha［J］. Nat Genet， 2007， 39（12）： 1500-1506.
23	JIANG S M， MINTER L C， STRATTON S A， et al. TRIM24 suppresses development of spontaneous hepatic lipid accumulation and hepatocellular carcinoma in mice［J］. J Hepatol， 2015， 62（2）： 371-379.
24	CHI J， YANG Q， XIE X F， et al. Clinical significance and prognostic value of TRIM24 expression in esophageal squamous cell carcinoma［J］. Aging （Albany NY）， 2016， 8（9）： 2204-2221.
25	ZHANG L H， YIN A A， CHENG J X， et al. TRIM24 promotes glioma progression and enhances chemoresistance through activation of the PI3K/Akt signaling pathway［J］. Oncogene， 2015， 34（5）： 600-610.

Parameter	n	Expression of TRIM24（Cytoplasm）		χ²	P	Expression of TRIM24（Nucleus）		χ²	P
Parameter	n	Low	High	χ²	P	Low	High	χ²	P
Age (year)
<60	41	23	18	0.294	0.587	16	25	4.054	0.044
≥60	38	19	19	0.294	0.587	7	31	4.054	0.044
Sex
Female	31	17	14	0.057	0.811	5	26	4.168	0.041
Male	48	25	23	0.057	0.811	18	30	4.168	0.041
Grade
Ⅰ/Ⅱ	68	39	29	3.441	0.064	21	47	0.253	0.615^a
Ⅲ/Ⅳ	11	3	8	3.441	0.064	2	9	0.253	0.615^a
T stage
T1/T2	75	41	34	0.415	0.519^a	23	52	-	0.316^b
T3	4	1	3	0.415	0.519^a	0	4	-	0.316^b
N stage
N0	78	42	36	-	0.468^b	23	55	-	1.000^b
N1	1	0	1	-	0.468^b	0	1	-	1.000^b
M stage
M0	77	42	35	-	0.216^b	23	54	-	1.000^b
M1	2	0	2	-	0.216^b	0	2	-	1.000^b
TNM stage
Ⅰ	58	33	25	1.220	0.269	19	39	1.404	0.236
Ⅱ/Ⅲ/Ⅳ	21	9	12	1.220	0.269	4	17	1.404	0.236
Tumor size (cm)
<6	48	25	23	0.057	0.811	18	30	4.168	0.041
≥6	31	17	14	0.057	0.811	5	26	4.168	0.041

Parameter (Cytoplasm)	Univariate analysis				Multivariate analysis
	HR	95%CI		P	HR	95%CI		P
	HR	Lower	Upper	P	HR	Lower	Upper	P
Expression of TRIM24 （High vs low）	3.353	1.552	7.243	0.002	3.523	1.520	8.168	0.003
Gender （Male vs female）	0.623	0.285	1.364	0.237	-	-	-	-
Age （≥60 years old vs <60 years old）	16.040	7.260	35.440	<0.001	0.975	0.221	4.304	0.974
Grade （Ⅲ/Ⅳ vs Ⅰ/Ⅱ）	25.040	5.859	107.000	<0.001	3.233	1.026	10.189	0.045
TNM stage （Ⅱ/Ⅲ/Ⅳ vs Ⅰ）	1.184	0.252	5.553	0.831	0.383	0.106	1.383	0.143
T stage （T3 vs T1/T2）	1.735	0.299	10.060	0.539	-	-	-	-
N stage （N1 vs N0）	22 . 929	33.870	15 .520 054	0.003	-	-	-	-
M stage （M1 vs M0）	2.723	0.167	44.380	0.482	-	-	-	-
Tumor size （≥6 cm vs <6 cm）	0.893	0.412	1.935	0.775	0.981	0.178	5.409	0.983

[1]	Fei WU,Qiwei YANG,Xin LI,Linxian ZHAO,Yujie SUI. Expressions of ANO1 and E-cadherin in human primary colorectal cancer tissue and their significances [J]. Journal of Jilin University(Medicine Edition), 2025, 51(6): 1638-1645.
[2]	Yu LIANG,Jinyu YU,Zhonggao XU,Wanning WANG. Expression characteristics of FOSB in kidney tissue from IgA nephropathy and other common kidney diseases [J]. Journal of Jilin University(Medicine Edition), 2025, 51(5): 1281-1292.
[3]	Ming CHEN,Qinghua LUO,Hongguang JIN,Liang HAN. Atypical lipomatous tumor of left lower limb: A case report and literature review [J]. Journal of Jilin University(Medicine Edition), 2025, 51(5): 1384-1389.
[4]	Ziru ZHOU,Mengfei SUN,Huakun ZHANG,Shuyan SUN,Qi SUN,Feng LI,Yunzhao CHEN,Jie YU,Yuwen CAO,Xiaobin CUI. Expression of Rh family C glycoprotein in esophageal squamous carcinoma and its clinical significance [J]. Journal of Jilin University(Medicine Edition), 2025, 51(4): 1019-1027.
[5]	Xialing HUANG,Dandan ZHANG,Xuemei ZHANG. Analysis on relationship between methylation of MSX1 gene and clinical pathological features and prognosis of patients with cervical cancer [J]. Journal of Jilin University(Medicine Edition), 2025, 51(3): 749-756.
[6]	Xiaoyan WANG,Xuelian LI,Bin LIANG,Wenfei TIAN,Hailin MA,Zhijing MO. Analysis on relationship between CALU and prognosis of hepatocellular carcinoma patients and its mechanism based on transcriptome and single cell sequencing data [J]. Journal of Jilin University(Medicine Edition), 2025, 51(2): 447-459.
[7]	Xi YANG,Qin YUAN,Lan YANG,Wenjie ZHANG. Bioinformatics analysis on PDE1B expression and prognosis of gastric cancer and tumor microenvironment [J]. Journal of Jilin University(Medicine Edition), 2024, 50(6): 1664-1676.
[8]	Jiaxin YI,Yangyu ZHANG,Yingli FU,Yuchen PAN,Yongjie HAN,Jing JIANG,Yanhua WU. Effect of MEIS1 expression on survival in patients after radical gastrectomy and its value in prognostic evaluation [J]. Journal of Jilin University(Medicine Edition), 2024, 50(5): 1358-1364.
[9]	Chaojie GUO,Jiajia ZHANG,Jie ZENG,Huiyu WANG, AIERFATI·Aimaier,Jiang XU. Expressions of PLOD1 in oral squamous cell carcinoma tissue and cells and their significances [J]. Journal of Jilin University(Medicine Edition), 2024, 50(4): 1035-1043.
[10]	Yuanguo WANG,Peng ZHANG. Bioinformatics analysis based on relationship between SSP1 and TGFB1 and occurrence, prognosis, and immune invasion of esophageal adenocarcinoma [J]. Journal of Jilin University(Medicine Edition), 2024, 50(4): 1076-1086.
[11]	Xiaoming WANG,Xueye WANG. Invasive adenocarcinoma of lung complicated with metastatic NUT midline carcinoma of mediastinal lymph node： A case report and literature review [J]. Journal of Jilin University(Medicine Edition), 2024, 50(3): 825-830.
[12]	Yueying SONG,Chao GAO,Wenjun CHEN,Aiyu SHAO,Yichun QIAO,Zhuolin LI. Screening of miRNAs related to prognosis of triple-negative breast cancer and its gene network based on TCGA Database [J]. Journal of Jilin University(Medicine Edition), 2024, 50(2): 392-399.
[13]	Yaqi XU,Yanyu WANG,Wenjing ZHANG,Mei HAN,Huaxia MU,Xi YANG,Weixiao BU,Zikun TAO,Yujia KONG,Fuyan SHI,Suzhen WANG. Bioinformatics analysis on screening of key genes of hepatitis B virus-related hepatocellular carcinoma and its relationship with prognosis [J]. Journal of Jilin University(Medicine Edition), 2023, 49(5): 1243-1252.
[14]	Haikang CUI,Xudong ZHANG,Xiaoning LI,Xi YANG,Lan YANG,Wenjie ZHANG. Bioinformatics analysis on predition effect of subtypes of cell pyroptosis and APOD on prognosis of gastric cancer patients [J]. Journal of Jilin University(Medicine Edition), 2023, 49(5): 1268-1279.
[15]	Jingjing LI, REZIWANGULI·Aisikaier,Wanyi XING,Yinggang ZOU. Primary dedifferentiated liposarcoma of unilateral ovary: A case report and literature review [J]. Journal of Jilin University(Medicine Edition), 2023, 49(4): 1040-1045.

Expression of TRIM24 protein in human clear cell renal cell carcinoma tissue and its clinical significance

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 25

Related Articles 15

Metrics

Comments

Recommended 10