Abstract:

Abstract:Objective
To investigate the influence of hedysaryum polysaccharide (HPS) in the kidney function and expressions of Glut-1 mRNA and protein in kidney tissue of db/db mice with diabetic nephropathy (DN) and to elucidate its possible action mechanism. Methods 10 db/m mice were taken as normal control group(n=10);50 fueling animal model db/db mice with DN were randomly divided into model group,enalapril group and the low,middle and high doses of HPS groups(n=10).The mice in noral control group and model group were given physioloical saline by gavege;and the mice in the other groups  were respectively given  10 mg•kg^-1•d^-1 enalapril,100,200 and 400 mg•kg^-1•d^-1 HPS by gavage;lasted 8 weeks. Picric acid method was used to determine the serum creatinine(SCr) level of the mice,enzyme coupling rate method was used to determine the blood urea nitrogen (BUN) level,ELISA method was used to determine the urinary microalbumin(UMALB) level, RT-PCR method was performed to detect the expression of Glut-1 mRNA,and Western blotting and mmunohistochemical methods were used to detect the expression of Glut-1 protein. Results Compared with model group,the levels of SCr,BUN,UMALB,the mRNA and protein of Glut-1 expressions were decreased,especially in 400 mg•kg^-1•d^-1 HPS and enalapril groups(P<0.01).The HE and Masson staining results showed that less inflammatory cells infiltration in glomerular of the mice were found,capillary lumens were unobstructed,and the collagen deposition was not obvious in 400 mg•kg^-1•d^-1 group. Conclusion HPS could improve the kidney function of the db/db mice  and inhibit the Glut-1 mRNA and protein expressions obviously,which indicates that HPS could delay the development of DN by inhibiting the Glut-1 expression in the glomerular mesangial cell membrane.
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Key words: hedysaryum polysaccharide, diabetic nephropathy, glucose tansporter protein-1, db/db mice