Abstract:

Abstract:Objective
To observe the influence of curcumin in the levels of  nitric oxide (NO) and S100βin the brain tissue of the  rats after cerebral ischemia reperfusion,and to explore the neuroprotective mechanism of curcumin.Methods Adult male SD rats were randomly divided into sham operation group,model group and curcumin group,and 18 rats in every group.Curcumin was intraperitoneally injected daily for 3 consecutive days before inducing focal cerebral ischemia in the SD rats by middle cerebral artery occlusion(MCAO) for 2 h.TTC staining was used to observe the infarction volume.Nitrate reductase assay was used to detect the level of NO in brain tissue of the rats.The level of S100β in brain was detected by ELISA method.Results Compared with model group,the  brain infarction volumes of the rats 24 and 72 h after cerebral ischemia reperfusion in curcumin group were significantly decreased (P<0.05).Compared with sham operation group,the NO and S100β levels in the brain tissue 24 and 72 h after cerebral ischemia reperfusion of the rats in model group were significantly increased(P<0.05);compared with model group,the levels of NO in the brain tissue 24 and 72 h after cerebral ischemia reperfusion in curcumin group were remarkably decreased (P<0.05）;compared with  modee group,the level of S100β in the brain tissue 72 h after cerebral iscemia reperfusion   in curcumin group was remarkably decreased  (P<0.05).Conclusion Curcumin can significantly reduce the degree of ischemia reperfusion injury in the rats and reduce the levels of NO and S100β in brain tissue,which suggests that the decrease of NO and S100β levels in brain tissue may be associated with the neuroprotective effect of curcumin.

Key words: curcumin, cerebral ischemia reperfusion, nitric oxide, S100&beta