骨保护素对大鼠正畸牙齿移动过程中破骨细胞分化和p38-MAPK信号通路的影响

doi:10.13481/j.1671-587x.20200618

Abstract

Abstract: Objective

To study the effects of osteoprotein on the osteoclast differentiation and p38-mitogen-activated protein kinase （p38-MAPK） signal pathway during the orthodontic tooth movement in the rats，and to explore the possible mechanism of osteoprotein in inhibiting the tooth movement during orthodontic treatment.

Methods

A total of 128 rats were randomly divided into control group and osteoprotectin group， and there were 64 rats in each group.Three days before modeling， the rats in osteoprotectin group were injected with recombinant human osteoprotectin （0.05 mg·kg^－1） submucosally on the left maxillary first molar，and the rats in control group were injected with the same amount of saline at the same site.The orthodontic models of rats were established by spring traction method.The moving distances of first molar of the rats at the 1st week，the 2nd week，the 3rd week， and the 4th week after stressing were measured.Tartrate-resistant acid phosphatase （TRAP） staining was used to determine the number of osteoclasts of the rats in two groups；immunohistochemical staining was used to determine the expression level of p-p38 on the pressure side of periodontal tissue of the rats in two groups；Western blotting method was used to determine the expression levels of receptor activators nuclear factor kappa B （RANK） and matrix metalloproteinase-9 （MMP-9） proteins in periodontal tissue of the rats in two groups.

Results

There were no significant differences in the tooth movement distances， osteoclast number and p-p38 expression levels of the rats between two groups at the 1st week after stressing； at the 2nd week， the 3rd week， and the 4th week after stressing， the tooth movement distances， osteoclast number and p-p38 expression levels of the rats in osteoprotegein group were lower than those in control group （P<0.05 or P<0.01）； the expression levels of RANK and MMP-9 proteins in alveolar bone tissue of the rats in osteoprotegein group were lower than those in control group （P<0.01）.The number of osteoclasts（r=0.654）， the expression levels of RANK（r=0.576） and MMP-9（r=0.583） proteins in periodontal tissue of the rats were positively correlated with the expression level of p-p38 protein（P<0.05）.

Conclusion

Osteoprotectin can inhibit the generation and differentiation of osteoclasts during the orthodontic tooth movement in the rats， and inhibit the activation of p38-MAPK signal pathway， thereby inhibit the orthodontic tooth movement and enhance the anchoring effect.

Key words: osteoprotectin, orthodontic treatment, tooth movement, osteoclasts, p38 mitogen activated protein kinase

CLC Number:

R783.5

Ying LOU,Xin MA. Effects of osteoprotectin on osteoclast differentiation and p38-MAPK signaling pathway during orthodontic tooth movement in rats[J].Journal of Jilin University(Medicine Edition), 2020, 46(6): 1221-1226.

Figures/Tables 6

Tab. 1

Tab.2

Tab.3

Fig. 3

Tab. 4

Tab.5

References 19

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Group	Movement distance
Group	(week) 1	2	3	4
Control	0.268±0.017	0.558±0.037	0.882±0.043	1.225±0.051
Osteoprotectin	0.256±0.015	0.481±0.032	0.741±0.037	0.983±0.045
t	1.497	4.452	7.030	10.064
P	0.157	<0.01	<0.01	<0.01

Group	Number of osteoclasts
Group	(week) 1	2	3	4
Control	3.08±0.31	3.61±0.28	5.10±0.34	3.74±0.21
Osteoprotectin	2.93±0.27	3.25±0.30	4.53±0.32	3.31±0.23
t	1.032	2.481	3.453	3.905
P	0.318	0.025	0.004	0.001

Group	Expression level of p-p38
Group	(week) 1	2	3	4
Control	4.903±1.137	15.247±1.203	22.135±1.346	18.694±1.275
Osteoprotectin	4.612±1.142	8.062±1.198	11.627±1.298	9.538±1.302
t	0.511	11.970	15.895	14.211
P	0.617	<0.01	<0.01	<0.01

Group	RANK				MMP-9
Group	(week)1	2	3	4	(week)1	2	3	4
Control	0.15±0.04	0.42±0.05	0.56±0.08	0.49±0.08	0.13±0.04	0.37±0.04	0.53±0.07	0.44±0.07
Osteoprotectin	0.11±0.04	0.18±0.03	0.38±0.06	0.34±0.06	0.10±0.03	0.21±0.02	0.36±0.06	0.31±0.05
t	2.000	11.642	5.091	4.243	1.697	10.119	5.215	4.274
P	0.064	<0.01	<0.01	0.01	0.110	<0.01	<0.01	0.001

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Effects of osteoprotectin on osteoclast differentiation and p38-MAPK signaling pathway during orthodontic tooth movement in rats

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Index	Expression level of p-p38
Index	r	P
Number of osteoclasts	0.654	<0.01
Expression level of RANK	0.576	<0.01
Expression level of MMP-9	0.583	<0.01