低剂量电离辐射对STZ诱导糖尿病模型大鼠海马神经元的影响

doi:10.13481/j.1671-587x.20210101

Abstract

Abstract: Objective

To investigate the effects of low dose ionizing radiation （LDR） on the cell cycle， calcium ion concentration（［Ca²⁺］i） and mitochondrial membrane potential（MMP） of hippocampal neurons in the diabetic rats， and to elucidate the protective mechanisms of LDR on nerve injury.

Methods

The Wistar rat models of diabetes mellitus （DM） were established with streptococci （STZ）.Sixty rats were divided into normal control group， DM X-ray irradiation group， DM+25 mGy X-ray irradiation group， DM+50 mGy X-ray irradiation group and DM+75 mGy X-ray irradiation group； there were 12 rats in each group. Except normal control and DM groups， the rats in other groups were administered with 25， 50 and 75 mGy LDR every other day for 12 times， and the body weights of the rats were monitored. After 4 weeks of feeding， the rats were sacrificed after anesthesia. The hippocumpus tissue of the rats in various groups was removed and single cell suspension was prepared. The percentages of hippocampal neurons at different cell cycles，（［Ca²⁺］i） and MMP were detected by flow cytometry， respectively.

Results

With the prolongation of time，the body weight of the rats in normal control group was increased gradually. Compared with normal control group， the body weights of diabetic rats in DM group， DM+25 mGy X-ray irradiation group， DM+50 mGy X-ray irradiation group and DM+75 mGy X-ray irradiation group were significantly decreased （P<0.05）； compared with DM group， the body weights in DM+50 mGy group and DM+75 group mGy group were significantly increased（P<0.05）. Compared with normal control group， the percentages of hippocampal neurons in S phase and ［Ca²⁺］i of the rats in DM group were increased significantly（P<0.05）， while MMP was decreased significantly （P<0.05）； compared with DM group， the percentages of hippocampal neurons in S phase and ［Ca²⁺］i of the rats in DM+50 mGy X-ray irradiation group and DM+75 mGy X-ray irradiation group were decreased significantly （P<0.05）， while MMP of hippocampal neurons was increased significantly （P<0.05）； compared with DM + 25 mGy X-ray irradiation group， the ［Ca²⁺］i in hippocampal neurons in DM+75 mGy X-ray irratiaiton group was decreased significantly（P<0.05）， while MMP of hippocampal neurons in DM+50 Gy X-ray irradiation group and DM+75 Gy X-ray irradiation group were increased significantly （P<0.05）.

Conclusion

LDR can improve the S phase delay， the increase of ［Ca²⁺］i and the decrease of MMP induced by DM of the hippocampal neurons of the rats to play the neuroprotective roles.

Key words: low dose radiation, diabetes mellitus, hippocampal neurons, cell cycle, mitochondrial membrane potential

CLC Number:

Q691

Fei LIU,Shuo LIANG,Yuexuan WANG,Lijing QIN,Wei GUO,Zhicheng WANG. Effect of low dose ionizing radiation on hippocampal neurons in STZ-induced diabetic rats[J].Journal of Jilin University(Medicine Edition), 2021, 47(1): 1-7.

Figures/Tables 5

Tab.1

Fig. 1

Tab.2

Fig. 2

Tab.3

References 22

1	BETLAZAR C， MIDDLETON R J， BANATI R B， et al. The impact of high and low dose ionising radiation on the central nervous system ［J］. Redox Biol， 2016， 9：144-156.
2	KOVALCHUK A， KOLB B. Low dose radiation effects on the brain-from mechanisms and behavioral outcomes to mitigation strategies ［J］. Cell Cycle， 2017， 16（13）：1266-1270.
3	SEONG K M， SEO S， LEE D， et al. Is the Linear no-threshold dose-response paradigm still necessary for the assessment of health effects of low dose radiation？［J］. J Korean Med Sci， 2016，31（）： S10-23.
4	AVERBECK D， SALOMAA S， BOUFFLER S， et al. Progress in low dose health risk research： Novel effects and new concepts in low dose radiobiology ［J］. Mutat Res， 2018， 776： 46-69.
5	KATAOKA T， YAMAOKA K. Activation of biodefense system by low-dose irradiation or radon inhalation and its applicable possibility for treatment of diabetes and hepatopathy ［J］. ISRN Endocrinol， 2012， 2012：292041.
6	EL-LATIF EI-GHAZALY M A， RASHED E R， SHAFEY G M， et al. Amelioration of thioacetamide-induced hepatic encephalopathy in rats by low-dose gamma irradiation ［J］. Environ Sci Pollut Res Int， 2020， 27（1）：334-343.
7	WANG B， TANAKA K， JI B， et al. Total body 100-mGy X-irradiation does not induce Alzheimer’s disease-like pathogenesis or memory impairment in mice ［J］. J Radiat Res， 2014， 55（1）：84-96.
8	TANG F R， LOKE W K. Molecular mechanisms of low dose ionizing radiation-induced hormesis， adaptive responses， radioresistance， bystander effects， and genomic instability ［J］. Int J Radiat Biol， 2015， 91（1）：13-27.
9	VOLPE C M O， VILLAR-DELFINO P H， ANJOS PMFDOS P M F， et al. Cellular death， reactive oxygen species （ROS） and diabetic complications ［J］. Cell Death Dis， 2018， 9（2）：119.
10	HANSEN J B， L R BDOS SANTOS， LIU Y， et al. Glucolipotoxic conditions induce β-cell iron import， cytosolic ROS formation and apoptosis ［J］. J Mol Endocrinol， 2018， 61（2）：69-77.
11	BAULCH J E， CRAVER B M， TRAN K K， et al. Persistent oxidative stress in human neural stem cells exposed to low fluences of charged particles ［J］. Redox Biol， 2015， 5：24-32.
12	SHIMURA T， SASATANI M， KAWAI H， et al. A comparison of radiation-induced mitochondrial damage between neural progenitor stem cells and differentiated cells ［J］. Cell Cycle， 2017， 16（6）：565-573.
13	MALEKIYAN R， ABDANIPOUR A， SOHRABI D， et al. Antioxidant and neuroprotective effects of lycopene and insulin in the hippocampus of streptozotocin-induced diabetic rats ［J］. Biomed Rep， 2019， 10（1）：47-54.
14	金百翰，宋敬云，谢俊豪，等. 血糖波动与氧化应激及细胞因子关系的研究进展[J]. 解放军医学杂志,2019,44(12): 1056-1060.
15	李芸菲, 孟凡华, 胡绮莉,等. 阿尔茨海默病患者选择性损害海马后部的MRI研究[J]. 同济大学学报(医学版), 2020,41(3): 348-352.
16	ZILLIOX L A， CHADRASEKARAN K， KWAN J Y， et al. Diabetes and Cognitive Impairment ［J］. Curr Diab Rep， 2016， 16（9）： 87.
17	YUAN X Y， WANG X G. Mild cognitive impairment in type 2 diabetes mellitus and related risk factors： a review ［J］. Rev Neurosci， 2017， 28（7）：715-723.
18	CALLISAYA M， NOSAKA K. Effects of exercise on type 2 diabetes mellitus-related cognitive impairment and dementia［J］. J Alzheimers Dis， 2017， 59（2）：503-513.
19	ORRENIUS S， GOGVADZE V， ZHIVOTOVSKY B. Calcium and mitochondria in the regulation of cell death［J］. Biochem Biophys Res Commun， 2015， 460（1）：72-81.
20	BRAVO-SAGUA R， PARRA V， LÓPEZ-CRISOSTO C， et al. Calcium Transport and signaling in mitochondria ［J］. Compr Physiol， 2017， 7（2）：623-634.
21	CHTOUROU Y， AOUEY B， KEBIECHE M， et al. Protective role of naringin against cisplatin induced oxidative stress， inflammatory response and apoptosis in rat striatum via suppressing ROS-mediated NF-κB and P53 signaling pathways ［J］. Chem Biol Interact， 2015， 239：76-86.
22	ZHOU Z， ZHOU B， CHEN H， et al. Reactive oxygen species （ROS） and the calcium-（Ca²⁺） mediated extrinsic and intrinsic pathways underlying BDE-47-induced apoptosis in rainbow trout （Oncorhynchus mykiss） gonadal cells ［J］. Sci Total Environ， 2019， 656：778-788.

Group	Body weight
Group	Before LDR	2 weeks of LDR	4 weeks of LDR	4 weeks after LDR
Normal control	211.0±10.7	260.8±10.5	324.0±19.1	524.0±15.1
DM	207.2±18.3	209.2±2.7^*	212.2±7.7^*	215.2±1.7^*
DM + 25 mGy X-ray irradiation	204.0±11.4	206.9±1.6^*	216.2±4.4^*	223.2±4.4^*
DM + 50 mGy X- ray irradiation	204.6±10.4	212.5±1.9^*	224.0±5.4^*	234.0±28.4^*△
DM + 75 mGy X- ray irradiation	199.2±7.3	209.5±2.7^*	223.8±5.8^*	238.8±5.8^*△

Group	Percentage of hippocampal neurons
Group	G₀/G₁	S	G₂/M
Normal control	87.6±3.8	3.9±0.4	8.6±0.6
DM	85.4±0.9	6.3±0.3^*	8.4±1.0
DM + 25 mGy X-ray irradiation	87.7±2.6	4.7±0.8^*△	7.6±1.4
DM + 50 mGy X-ray irradiation	87.2±0.8	3.5±0.4^*△	9.7±0.9
DM + 75 mGy X-ray irradiation	86.4±2.5	4.5±0.6^*△	9.1±0.9

Group	[Ca²⁺] i	MMP
Normal control	9.4±0.9	31.3±4.3
DM	14.7±1.5^*	25.1±1.4^*
DM+ 25 mGy X-ray irradiation	14.8±0.5^*	25.8±1.0^*
DM+ 50 mGy X-ray irradiation	12.2±0.6^*△	30.5±1.6^△#
DM+ 75 mGy X-ray irradiation	6.5±0.4^*△#	29.1±0.1^△#

[1]	Jing YU,Yinzhuo HUANG,Zhongwei ZHOU,Yuxin XIE,Qian MAO. Analysis on influencing factors in type 2 diabetes mellitus patients complicated with lower limb atherosclerosis and construction of nomogram model [J]. Journal of Jilin University(Medicine Edition), 2026, 52(3): 806-812.
[2]	Shu LI,Jiaqi GUO,Wansong LI,Yanfeng ZHEN,Hongjia ZHAI,Jie LI,Hui FANG. Improvement effect of fingolimod on hepatic fibrosis in type 2 diabetes mellitus mice and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2026, 52(2): 330-339.
[3]	Shanshan SUN,Mei LU,Xinfu GAO,LYu Guangyao,Baolei Zhao,LYu Wenwen. Inhibitory effect of Bradykinin 1 receptor antagonist ELN441958 on proliferation of HepG2 cells by regulating Akt/FoxO3a signaling pathway [J]. Journal of Jilin University(Medicine Edition), 2026, 52(1): 70-80.
[4]	Dayou DAI,Zhigang ZHANG,Hui LI. Effect of CHRNA5 in occurrence and development of pancreas cancer and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2025, 51(6): 1518-1531.
[5]	Shuang LI,Jiayu GE,Xianzhu CONG,Aimin WANG,Yujia KONG,Fuyan SHI,Suzhen WANG. Analysis on influencing factors for occurrence of angina pectoris in diabetic mellitus patients and its Bayesian network risk prediction [J]. Journal of Jilin University(Medicine Edition), 2025, 51(4): 1028-1038.
[6]	Yixuan GAO,Peng WANG,Silong ZHANG,Ruijuan GAO,Yingfang MA,Keke ZHANG,Dan FENG,Zongqi HUANG,Ketao MA,Li LI,Junqiang SI. Inhibitory effect of safranal on proliferation, migration and phenotypic transformation of vascular smooth muscle cells of rats induced by high glucose in vitro [J]. Journal of Jilin University(Medicine Edition), 2025, 51(4): 948-957.
[7]	Chao LIANG,Juanjuan DAI,Ning ZHOU,Dandan WANG,Jie ZHAO,Di AN,Yan WU. Effect of oridonin on cell proliferation, migration， and apoptosis of human nasopharynx carcinoma HONE-1 cells [J]. Journal of Jilin University(Medicine Edition), 2024, 50(4): 917-924.
[8]	Jiacai FU,Lingsha QING,Lu YANG,Meihui SONG,Xianying ZHANG,Xiaocui LIU,Fengjin LI,Ling QI. Inhibitory effect of Schisandrin B on proliferation of pancreatic cancer Pan02 cells and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2024, 50(3): 638-646.
[9]	Xue FANG,Yu KANG,Xiaoxi SHA,Zhifen HAN,Yan ZHANG. Detection of gastrocnemius muscle hardness of diabetic patients under different functional states by ultrasonic shear wave elastography and its clinical significance [J]. Journal of Jilin University(Medicine Edition), 2024, 50(2): 451-456.
[10]	Zhongxin FENG,Mei LI. Effect of soluble CD40 ligand on biological behavior of THP-1 cells through long non-coding RNA linc00239 [J]. Journal of Jilin University(Medicine Edition), 2024, 50(1): 88-96.
[11]	Lu FU, Yanjue YE, Jiangying LI, Ziyi TANG, Li YIN. Expressions of Sirtuins protein in testis tissue and GC-2 cells in male reproductive system damage model mice induced by bisphenol A and their significances [J]. Journal of Jilin University(Medicine Edition), 2023, 49(5): 1107-1116.
[12]	Jiaxin WANG,Zhenqi WANG,Xuan ZHANG. Expressions of CDKAL1 gene and its splice isomers in peripheral blood lymphocytes of patients with type 2 diabetes mellitus and their clinical significances [J]. Journal of Jilin University(Medicine Edition), 2023, 49(5): 1290-1295.
[13]	Haiyao PANG,Li HOU,Wenning HE,Jun MENG. Expression levels of serum and glucocorticoid-induced protein kinase 3 in one-cell stage fertilized eggs at different cell cycles in mice and its subcellular localization [J]. Journal of Jilin University(Medicine Edition), 2023, 49(4): 884-889.
[14]	Hongli CUI,Siqi FAN,Wenfei GUAN,E MENG,Jiatong LIU,Xuetong SUN,Chunxu CAO,Lixin LIU,Yali QI,Fang FANG,Zhicheng WANG. Inhibitory effect of irradiation enhanced by gallic acid-lecithin complex-induced oxidative stress on proliferation of A549 cells [J]. Journal of Jilin University(Medicine Edition), 2023, 49(4): 941-946.
[15]	Naixu SHI,Miao HAO,Tianfu ZHANG,Kelin ZHAO,Ziyan HUANG,Chunyan LI,Xiaofeng WANG. Inhibitory effect of baicalein on proliferation of human tongue squamous cell carcinoma CAL27 cells and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2023, 49(4): 985-993.

Effect of low dose ionizing radiation on hippocampal neurons in STZ-induced diabetic rats

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 22

Related Articles 15

Metrics

Comments

Recommended 10