Abstract: Abstract:Objective   To study the differential expression of miRNAs and their target genes in papillary thyroid carcinoma（PTC）tissue,and to analyze the potential roles of miRNAs as biomarkers and in carcinogenesis of PTC. Methods The PTC samples and their matching normal thyroid tissues were examined and collected.The genes and miRNA expression profiles were examined with Illumina Bead Chips.KEGG pathway was used to analyze the function of differential expression genes.MiRNA target genes were predicted by implementing three computational analysis programs,the results were verified by qRT-PCR.Results 248 miRNAs and 3 631 genes were found to be differentially expressed(gene:P<0.05,miRNA:P<0.01) in PTC tissues as compared with their matching normal thyroid tissues.Hsa-miR-101（target gene:ITGA3）was identified.The hsa-miR-101 expression in PTC tissue(59.8%) was down-regulated and ITGA3s expression (100%)  was up-regulated compared with their matching normal thyroid tissues.Conclusion ITGA3 may be the  target gene of hsa-miR-101,and both of them may play important roles in occurrence and development of PTC.

Key words: thyroid neoplasms, MicroRNA, biomarker, gene profile