Abstract: Abstract:Objective To explore the therapeutic effect of suppository of Jiechang An combined with  Shuxue Ning on rats with ulcerative colitis(UC) induced with TNBS  and to clarify its mechanisms of Huoxue,anti-inflammtory,and immunomodulation.Methods 64 Wistar rats were randomly divided into control group(n=8),model group(n=8),mesalazine group(0.380 g/kg,n=8),Shuxue Ning group(1.8 mL?kg-1,n=8),suppository of Jiechang An  A and B  groups(1.215 g/kg，2.430 g/kg,n=8),suppository of Jiechang An combined Shuxue Ning A and B groups（1.215 g/kg+1.8 mL/kg and 2.430 g/kg+1.8 mL/kg,n=8).The UC model was induced by TNBS in rats.The drugs were administrated for 14 d after  modelling.The symptomatology,body weight,blood index,and proliferation ability of lymphocytes were observed.The expression levels of IFN-γ and TNF-α mRAN were detected with RT-PCR.The  pathological changes of colon tissue of rats  and the expression  of CD4+ in spleen tissue of rats were examined  by HE staining and  immunohistochemical staining. Results  Compared with model group,the symptoms  were improved  in combined drug groups,the body weights and stimulation index(SI) were increased;the platelet (PLT) amount(P<0.05 or P<0.01),the number of three kinds of inflammatory cells,the expression levels of IFN-γ，TNF-α mRNA  were decreased in combined drug groups;but the prothrombin time (PT)and partial thromboplastin time (APTT) were prolonged in combined drug groups（P<0.05 or P<0.01）.Obvious   colonic mucosa damages，such as  erosion and  ulcer formation of colonic mucosa and  a large number of infiltrated inflammatory cells  in model group were found. However,the colonic mucosa damage and inflammatory infiltrating were much slighter;parts of colonic mucosa ulcer mucous membrane epithelium was repaired,and the bodies of gland arranged in good order relatively, and the  damaged cyathiform cells were decreased in combined drug groups. Immunohistochemical staining showed that the numbers of cells with positive CD4+expression  in  combined drug groups were decreased.Conclusion Suppository of Jiechang An combined with  Shuxue Ning has therapeutic effect in UC rats,and its mechanism may be related to reducing inflammatory reaction through Huoxue and immunomodulation.

Key words: suppository of Jiechang An, ShuxueNing, ulcerative colitis, immune, inflammatory