Abstract:

Abstract:Objective
To study the induction of cisplatin on apoptosis of human glioma SHG-44 neurospheres,and to explore its mechanism.Methods The SHG-44 neurospheres were cultured and divided into control and cisplatin groups.The cells in  control group were treated with stem cells media,and based on control group,the cells in cisplatin group were treated with 10 mg•L^-1 cisplatin for 24,48 and 72 h.MTT assay was used to evaluate the  inhibitory rate of proliferation of SHG-44 neurospheres;the morphology of the  cells  was observed by inverted microscope;the levels of Bax,Bcl-2,and caspase-3 proteins secreted by SHG-44 neurospheres  were detected by ELISA assay.Results Compared with control group,the  inhibitory rates of SHG-44 neurospheres in cisplatin group were increased  after treated for 24,48 and 72 h(P<0.01).The apoptotic bodies were observed and the number of neuroshperes were reduced in cisplatin group  under inverted microscope.Compared with control group,the levels of Bax and Bcl-2 were decreased,the level of caspase-3  was increased,and the ratio of Bax/Bcl-2  was significantly increased in cisplatin group (P<0.05 or P<0.01).Conclusion Cisplatin could significantly inhibit the growth of SHG-44 neurospheres and induce apoptosis,and its mechanism may be related to  up-regulating the ratio of Bax/Bcl-2 and giving the apoptosis factors the advantage.

Key words: cisplatin, glioma, neurospheres, apoptosis, Bax/Bcl-2