Abstract:

To construct the recombinant eukaryotic expression vector of mitochondrial superoxide dismutase 2 (SOD2) and to explore its expression efficacy in human umbilical vein endothelial cells (HUVEC).Methods SOD2 gene full-length cDNA was amplified from HUVEC by RT-PCR and the target gene SOD2 was connected with the plasmid pcDNA3.0 vector.The recombinant plasmid was constructed and transfected into HUVEC.The cells were grouped into HUVEC,pcDNA3.0 and pcDNA3.0-SOD2 transfected groups.The expression of SOD2 in HUVEC was detected by RT- PCR.

Key words:  , mitochondrial superoxide dismutase, pcDNA3.0, human umbilical vein endothelial cell