Abstract:

Abstract:Objective To investigate the expressions of MDR1 and P-glucose protein (P-gp) in brain tumor stem cells(BTSCs),and find new therapeutic targets and better anticancer strategies.Methods Tumor samples were obtained from consenting patients,actutely dissociated,triturated into single cells,and then inoculated into serum-free medium.After the primary brain tumor spheres generated,they were triturated again and passaged into fresh medium.Finally,they were digested into single cells with dispase.The specific marker CD133 was detected with immunofluorescent method.The expression of MDR1 in BTSCs was determined with immunohistochemical method,and the expression of P-gp was detected with Western blotting.Results The BTSCs isolated from glioblastoma,had the ability to propagate and differationate in vitro.The expression of CD133 was proved by immunocyt ochemistry and immunofluorescent method,the same with the MDR1,and the expression rate of MDR1 in the BTSCs was (45.3±5.4)%.P-gp expressed in BTSCs detected by Western blotting.Conclusion There are surely MDR1 and P-gp  expressions in BTSCs,which may make them more resistant to chemical therapy.

Key words: neoplastic stem cells;brain;multi-drug resistance gene;P-glycoprotein