Abstract:

Abstract:Objective To study the effect of staphylokinase derivation (SakD) on the function of platelet in hyprlipemia rats and its mechanism. Methods 30 Wistar rats were randomly divided into 3 group: SakD group, hyperlipemia group and  normal diet group (n=10). The rats in SakD group received SakD i.v. for 15 times (0.5 mg?kg-1, once every other day) respectively, and the rats in other  two groups received normal saline in the same way after 8 weeks feeding. The blood samples were collected before administration and 24 h after the last injection. Then the platelet adhesion rate and the adenosine diphosphate(ADP)-induced platelet aggregation rates in various groups were detected, the contents of calmodulin (CaM) and thromboxane(TXA2)in platelet were measured with radioimmunoassay. Results Compared with hyperlipemia group,the adhesion platelet function and ADP-induced platelet aggregation of hyperlipemia  rats in SaKD group was significantly decreased(63.5%±6.71% and 44.7%±8.13%,  respectively, P<0.05;91.56%± 9.01% and  81.22%±3.45%, P<0.05), the releasing of platelet TXA2 and CaM content in platelet were significantly reduced (P<0.05 or P<0.01) Conclusion SakD can suppress  the platelet agglutination and adhesion by intervention of different pathways of platelet activation.

Key words: staphylokinase derivation, platelet, hyperlipemia