Abstract:

Abstract:Objective To explore the possibility to damage mice pancreas by recombinant fusion protein vaccine,heat shock protein 65-mucin 1 (HSP65-MUC1) via molecular mimicry.Methods 21 C57BL/6 mice were randomly divided into three groups:PBS control,HSP65 and HSP65-MUC1 groups.The mice in each group were injected underneath with PBS,HSP65 and HSP65-MUC1,  respectively,once a week for three weeks. The mouse  pancreas were isolated under aseptic situation,the proliferation of specific lymphocytes was   tested by flow cytometry,the pathological changes of mouse pancreas were detected with histo-pathological analysis. Results By flow cytometry,compared with   PBS and HSP65 groups,the lymphocytes specific to HSP65-MUC1 of mice in HSP65-MUC1 group were increased by 16.88%（P<0.001）,the lymphocytes specific to HSP65 were increased by 7.29 %（P<0.01）;the lymphocytes specific to HSP60 were increased by 5.79 %（P<0.05). However,HE staining of mouse pancreas in immunized mice kept normal. Conclusion The mouse pancreas are not injured by HSP65-MUC1 via molecular mimicry.

Key words: recombinant fusion protein anti-tumor;vaccines;heat shock protein 65-mucin1;molecular mimicry