Abstract:

Abstract:Objective
To observe the vascularization of muscle basal lamina after transplantated into rat spinal cord injury,and to provide  theoretical and experimental basis for the use of muscle basal lamina as tissue engineering material in spinal cord injury. Methods  Muscle basal lamina was made by the frozen-thawed process and transplanted into the rat hemisected spinal cord  model. The rats were sacrificed on the 3，5，7，14 and 28 day after the operation. The vascularization of scaffolds was observed at different time points by alkaline phosphatase staining(APS). The number of blood vessels in scaffold was compared at different time points(3,5,7,14 and 28 d). Results After transplantation,the newly formed blood vessels could grow into the muscle basal lamina scaffold from the juntional area between the scaffold and spinal cord to the inside of scaffold,and a network in the scaffold was formed. The percentage of blood vessel area increased gradually and the differences between each two groups were statistically significant, 5 d > 3 d (P<0.05);7 d >5 d (P<0.01);14 d>7 d ( P<0.01);28 d>14 d (P<0.01). Conclusion Muscle basal lamina has better  vascularization after transplantation into the rat spinal cord injury model and it is expected to become suitable tissue engineering material to repair spinal cord injury.

Key words: muscle basal lamina;hemisection spinal cord injury;angiogenesis