Abstract:

Abstract:Objective
To observe and compare the photochemotherapeutic effects of BCPD-17 (hematoporphyrin derivative) and PSD-007 on osteosarcoma LM-8 transplanted in mice and to explore a novel photosenitizer for photodynamic therapy of  osteosarcoma Methods The tumor model was made by subcutaneous injection of osteosarcoma LM-8 on C3H mice.When the tumors reached about 6-8 mm in diameter,the tested mice were randomly divided into control group,PSD-007 group (5 mg•kg^-1),BCPD-17 groups (5 and 10 mg•kg^-1).After received intravenous injection of 5 or 10 mg•kg^-1 of PSD-007 and BCPD-17,respectively,the tumors were irradiated vertically with 630 nm or 670 nm laser (power density 200 mW•cm-2,energy density 240 J•cm^-2) for 20 min.The size of osteosarcoma was measured 7 d  after treatment.And all the tumors were extirpated,weighted and taken for pathological examination.The inhibitory rate of tumor was calculated.Results The results of pathohistological observation showed that in control group the size of mouse osteosarcoma was big and hyperchromatic with obvious nuclear atypia;in PDT treatment group,the turmor cells presented apoptosis,vacuolar degeneration and karyopycnosis,especially in BCPD-17 group. Compared with control group,the volumes and the weights of the tumors in PDT treatment groups were obviously decreased,there were significant differences(P<0.01).Compared with PSD-007 group,the inhibitory rates of tumor in BCPD-17 groups irradiated by the laser with wavelength 670 nm were increased,there were significant differences(P<0.01).Conclusion Both of the photosenitizers BCPD-17 and PSD-007 could obviously inhibit the growth of the tumors.Photosenitizer BCPD-17 has more anti-tumor effect.

Key words: photodynamic therepy, LM-8 osteosarcoma , BCPD-17 ;PSD-007;inhibitory rate of tumor