Abstract:

Abstract:Objective
 To expiore the influence of  oral low dose of mixed rare earth　Changle in the  morphological structure of the kidney of diabetic rats, and clarify its repair on  kidney structure damage of diabetic rats.Methods  Male Wistar rats were treated with STZ to set up diabetes mellitus models and then  divided into  diabetic model group and Changle treatment group (n=10),the other 10 nomal rats were used as control group,the rats in three groups were given daily saline, Changle(0.2 mg?kg-1),and daily saline,orally one month, then they were executed and the kidney was obtained.The　kidney morphometry was used to detect the changes  of  rat kidney weight, body weight
, kidney weight to body weight ratio, renal cortical thickness, glomerular volume density, total glomerular volume, near (far) end of the total volume, near (far) end of the tubular epithelial cell height, near (far) end of tube cavity diameter  and the morphological changes of kidney.Results  Compared with control group and Changle treatment group,in diabetic model group, the kidney weight was increased significantly(P<0.01), the body weight was markedly decreased (P<0.01),the kidney weight to body weight ratio was significantly increased(P<0.01);the total glomerular volume, near (far) end of a small tube volume, proximal tubule epithelial cell height, near (far) end of  the small tube cavity diameter were significantly increased  (all P<0.01); the kidney cortical thickness, glomerular volume and diatal tubule epithelial cells had no significant change(P>0.05).Compared with control group, in Changle treatment group the kidney weight, renal cortical thickness and glomerular volume density, glomerular total volume, total vovlume of proximal tubule, near (far) end of the tubular epithelial cell height, near (far) end of tube cavity diameter had no significant difference(P>0.05), but the body weight was significantly reduced (P<0.01), and the kidney weight to body weight  ratio,near (far) end of a small tube,and  total volume were significantly increased  (P<0.01).The HE staining results showed that the kidneys in control  and Changle treatment groups were normal, the rat kidney in diabetic model group was swollen;the  PAS staining results showed that in diabetic model group most of the proximal tubular epithelial cells were markedly swollen, containing increased PAS positive material, a small number of  proximal tubules showed the shedding of epithelial cells, lumen expansion and part of expansion of the distal tubule; however there were no significant changes in epithelial cells;in normal control group and Changle treatment group there were no the  changes mentioned above.Conclusion Oral low dose of  mixed rare earth Changle can repair  structure damage of  the kidney of the  diabetic rats.

Key words: Changle, diabetes mellitus, kidney, morphometry