Abstract: Abstract:Objective To detect the expressions of SHIP mRNA and Caspase-3 mRNA in bone marrow tissue of  the patients with   myelodysplastic syndrome （MDS） and to discuss the relationship between SHIP mRNA and Caspase-3 mRNA expressions and the occurrence and development of MDS.Methods 20 low-risk MDS cases(low-risk group)，12 Int-1 MDS cases(Int-1 group),13 Int-2 MDS cases(Int-2 group),and 17 high-risk MDS cases(high-risk group) were selected according to International Prognostic Scoring Syterm(IPSS).30 healthy volunteers were regared as control group.The expression levels of SHIP mRNA and Caspase-3 mRNA in bone marrow mononuclear  cells (BMNC) in various groups were detected by RT-PCR method,and the relationship between them was analyzed. Results The positive rates of SHIP mRNA were 100% and 100% and its expression levels were 4.467±2.899 and 4.529±1.975 in low-risk group and Int-1 group,respectively,while they were 100% and 4.623±2.979 in control group,so there were no significant differences between low-risk group,Int-1 group and control group (P>0.05).The positive rates of SHIP mRNA were 90% and 88% and the expression levels were 2.124±1.880 and 2.574±1.807 in Int-2 group and high-risk group,respectively,so there were significantly differences compared with control group (P<0.05).The Pearson correlation analysis results showed that the positive correlations were found between the level of SHIP mRNA and the level of Caspase-3 mRNA in MDS patients(r=0.714，P>0.05).The expression level of SHIP mRNA was decreased with the increasing of premitive cells(F=3.7，P<0.05).There were no significant differences of SHIP mRNA expression levels of patients between different genders(t=0.940，P>0.05) and different ages(t=1.417，P>0.05).Conclusion The expression level of SHIP  is lower or the SHIP doesn’t  express in  BMNC of the high-risk MDS patients, and  its expression level   can be used as an indicator to judge the prognosis of MDS.
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Key words: myelodysplastic syndrome, gene expression, SH2 domain-containing inositol 5&rsquo, -phosphatase, polymerase chain reactiom