Abstract:

Objective To explore the association of low molecular weight protease
( LMP) gene single nucleotide polymorphisms (SNP) with the susceptibility of pulmonary tuberculosis (PTB) of Li population in Hainan Province,and to clarify the LMP gene polymorphism in Li population,and to provide theoretical basis for research on genomics.Methods
Two polymorphicloci of LMP2/LMP7 (Codon60/Codon145/) in 205 cases of Li population  in Hainan with PTB(case group) and 217 healthy Li population in Hainan(control group)  were detected with PCR-RFLP,and the susceptibilities were   analyzed. Results  The polymorphic loci of the LMP gene had polymorphisms in two groups,and LMP7 were C→A,LMP2 were C→T.The genotypic frequencies of  homozygous  Lys/Lys and  heterozygous  Gln/Lys of LMP7 Codon145 polymorphic loci in case group were significantly higher than those in  control group.The genotypic frequencies of  Gln/Gln and homozygous  Lys/Lys were compared,OR
=3.77 (95%CI:1.60-8.89,P<0.05);compared with  Gln/Lys,OR=2.97 (95%CI:1.80-4.90,P<0.01).The genotypic frequency of LMP2 gene polymorphic loci had no significant difference between two groups (P>0.05).The polymorphism of LMP7 gene  was a predisposing factor  for pulmonary tuberculosis infection
  of Li population in Hainan,and the homozygous and heterozygous forms increased the tuberculosis risk for 3.77 and 2.97 times.
Conclusion For the infection of Mycobacterium tuberculosis,LMP7 gene polymorphism is still an important predisposing risk factors.

Key words: pulmonary tuberculusis, single nucleotide polymorphism, low molecular weight proteasome, Li polulation