Abstract:

Objective To build a new type of wear particle induced bone dissolution model,and to provide a simple and convenient aseptic loosening experimenta
l animal model for the study of artificial joints.Methods A total of 50 BALB/c mice were randomly divided into blank control group,pure salt group(n＝10),airbag bone graft group(n＝10),and salt embedding wear particle group(n＝10).The skull bone samples of mice in airbag bone graft group and other groups were obtained 21 and 14 d after operation,respectively.The number of inflammatory cells and osteolysis area were observed  by HE staining.The percentage of bone absorption lacuna area was detected by scanning electron microscope.The proliferation and differentiation of osteoclasts were observed by TRAP staining Results The HE staining results showed that compared with airbag bone graft group and black group,  the efflux of inflammatory cells and osteolysis areas in pure salt group and wear particle group were increased significantly (P<0.01). The TRAP  staining results showed that the number of osteoclasts in airbag bone graft group was significantly lower than that in  wear particle group (P<0.01).The scanning electron microscope results  showed that the percentage of bone absorption lacuna area in  wear particle group was significantly higher than that in  airbag bone graft group (P<0.01).Conclusion Salt solution  method is a simple,economic,and fast method to build myuse skull dissolvement  models,and  it can accurately simulate the pathological process of aseptic loosening after artificial joint replacement.
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Key words: wear particle, skull dissolution, aseptic loosening;air pouch model；mice,inbred BALBc