Abstract:

Abstract:Objective To observe the effect of NDGA on the survival of  amniotic epithelial cells(AECs) in the chemical acellular muscle scaffold after transplanted into injured spinal cord,and to provide experimental basis for the application
of DNGA in spinal cord injury. Methods  Chemical acellular muscle scaffold combined with Dil labeled AECs of 6 pregnant  rats were  used for spinal cord hemisection injury model. 72 adult male rats  were randomly divided into saline group(n=18),20 mg•kg-1NDGA group(n=18),30 mg?kg-1NDGA group(n=18),and 40 mg•kg-1NDGA group (n=18).The rats in saline group were given intraperitoneal injection of saline. The rats in each NDGA  groups were given intraperitoneal injection of NDGA by the  concentration of 20,30,and 40 mg•kg-1,respectively. And 6 rats in  each group  were separately sacrificed on the 1st,2nd,and 4th week  after operation. The number of the survival AECs waas observed under  fluorescence microscope; the  percentage of positive blood vessel area and the number of NG2 positive cells of the rats in various groups  were observed by immunohistochemical method. Results The number of AECs of the rats in various groups was decreased with the prolongation of time.1,2,and 4 weeks after transplantation,the number of AECs  in  30 mg•kg-1NDGA group and 40　mg?kg-1NDGA grou
p was significantly higher than those in  saline group and 20 mg?kg-1NDGA group (P<0.05). 1 week after transplantation,the  percentages of positive blood vessel area of the rats in  30 mg•kg-1NDGA group and 40 mg•kg-1 NDGA group were significantly lower than those in  saline group and 20 mg•kg-1NDGA group (P<0.05). 1 week after transplantation,the number of the NG2 positive cells of the rats  had no significant difference between various  groups (P＞0.05).Conclusion
NDGA is  able to significantly extend the survival of the transplanted AECs in the chemical acellular muscle scaffold after spinal cord injury. NDGA  can inhibit the new blood vessels of the injured spinal cord,and dose not affect the endogenous neural stem cells.

Key words: NDGA, chemical acellular muscle scaffold, amniotic epithelial cells, spinal cord injury