Abstract:

Objective To explore  the differences of the  expressions of MMP-9 and
 TIMP-1 mRNA in  esophageal squamous cell carcinoma tissue and the matched adjacent normal tissue
 and their relationships. Methods 98 spicemens of surgical removed esophageal carcinoma tissue were chosen for detection,the carcinoma tis
sue and the  adjacent normal tissue  were cut and stored simultaneously from every spicemen,respectively.RT-PCR method was used to detect the expressions of MMP
-9 and TIMP-1 mRNA in  esophageal squamous cell carcinoma tissue and the  adjacent normal tissue;the relationships between the expressions of MMP-9 and
TIMP-1 mRNA in esophageal squamous cell carcinoma tissue and clinicopathological characteristics were analyzed.The relationship between the expressions of MMP
-9 and TIMP-1 mRNA in carcinoma tissue was analyzed by Pearson related analysis method.Results
The positive expression rate of MMP-9 mRNA in carcinoma tissue was higher than that  in    adjacent normal tissue
(χ2=22.873，P=0.000), and the positive expression rate of TIMP-1 mRNA in carcinoma tissue was higher than that
 in   adjacent normal tissue （χ2=7.864，P=0.005）.There was  correlation  between the positive expr
ession of MMP-9 mRNA and the depth of tumor infiltration（χ2=5.022，P=0.025）, but there was no correlation  between the positive expression of MMP-9 mR
NA and lymph node metastasis（χ2=0.617，P=0.432）.There was  correlation  between the positiv
e expression of TIMP-1 mRNA and the depth of tumor infiltration（χ2=4.982，P=0.026）, but there was  no correlation  between the positive expression o
f TIMP-1 mRNA and lymph node metastasis（χ2=0.527，P=0.468）. The  positive expression of MMP-9 mRNA  in
esophageal  carcinoma tissue had positive correlation with the expression of TIMP-1 mRNA（r=2.237，P=0.028）.
Conclusion The expressions of MMP-9 and TIMP-1 mRNA may play a very important role in  the development of esophageal squamous cell carcinoma,however both of them have no affection on lymph node metastasis of esophageal squamous cell carcinoma.
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Key words: esophageal squamous cell carcinoma, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1