Fifty BALB/c mice were randomly divided into control group， model group， and low， medium and high doses of honeysuckle extract groups（n=10）.The mice in low， medium and high doses of honeysuckle extract groups were given 250， 500 and 750 mg·kg^－1 honeysuckle extract by gavage， and the mice in control group and model group were given normal saline. After administration for 15 d，the mice in other groups except control group were injected with 125 mg·L^－1 LPS in the vitreous cavity to establish the AAU mouse models. HE staining was used to observe the pathomorphology of the eye tissue of the mice.Real-time fluorescence quantitative PCR（RT-qPCR） and Western blotting methods were used to detect the expression levels of Toll-like receptor 4（TLR4） and nuclear factor-κB（NF-κB） mRNA and proteins in eye tissue of the mice.ELISA was used to determine the levels of tumor necrosis factor-α （TNF-α）， interleukin-6 （IL-6） and interleukin-10 （IL-10） in eye tissue of the mice.

The eye tissue of mice in control group was intact and had no inflammatory cell infiltration. Retinal edema occurred and a large number of inflammatory cells infiltrated in tissue of the mice in model group. Compared with model group， the infiltration of inflammatory cells in uvea tissue of the mice in low， medium and high doses of honeysuckle extract groups were decreased to different degrees.Compared with control group， the levels of TLR4 and NF- κB mRNA and proteins in model group were significantly increased （P<0.05）， while the levels of TNF-α， IL-6 and IL-10 were significantly increased （P<0.05）. Compared with model group，the levels of TLR4 and NF-κB mRNA and proteins in low， medium and high doses of honeysuckle extract groups were significantly decreased （P<0.05）， while the levels of TNF-α， IL-6 and IL-10 were significantly decreased （P<0.05）. Compared with low dose of honeysuckle extract group，the levels of TLR4 and NF-κB mRNA and proteins in eye tissue of the mice in medium and high doses of honeysuckle extract groups were significantly decreased （P<0.05），while the levels of TNF-α， IL-6 and IL-10 were significantly decreased（P<0.05）. Compared with medium dose of honeysuckle extract group，the levels of TLR4 and NF-κB mRNA and proteins in high dose of honeysuckle extract group were significantly decreased （P<0.05）， while the levels of TNF-α， IL-6 and IL-10 were significantly decreased（P<0.05）.

Honeysuckle extract can down-regulate the expression of inflammatory cells and reduce the inflammatory response of eye tissue in the AUU mice through the TLR4/NF-κB signaling pathway.