Abstract:

Objective
To investigate the genetic association between the polymorphism of rs12026099 site,rs2250312 site and rs1022528 site on PTGER3 gene and schizophrenia in Han population from the north of China.Methods PCR-RFLP analysis was employed to detect the genotype of PTGER3 gene in 240 family trios consisting of fathers,mothers and affected offsprings with schizophrenia.Haplotype relative risk (HRR) analysis,transmission disequilibrium test (TDT),Haplotype analysis and association between SNPs and clinical symptoms analysis were used to analysis the genotype data.
Results The genotypic frequency of the PTGER3 gene did not deviate from Hardy-Weinberg equilibrium in both patient group and parent group (P>0.05);HRR analysis did not show allelic association with the PTGER3 gene (χ2= 1.396,P>0.05;χ2= 0.042,P>0.05;χ2= 0.119,P>0.05);TDT analysis did not show preferential transmission of the two alleles (χ2= 1.380,P>0.05;χ2= 0.043,P>0.05;χ2= 0.126,P>0.05);Haplotype anal
ysis showed that the two SNPs were in one haplotype block,but the haplotype was not associated with schizophrenia(χ2=6.500,P>0.05);χ2 test for association betwee
n SNPs and clinical symptoms of schizophrenia showed that rs12026099 locus was associated with apathy of schizophrenia and illogic thought of paranoid type schizophrenia (χ2=4.578,P=0.032;χ2=6.100,P=0.047),rs2250312 locus was associated with illogic thought and abulia of paranoid type schizophrenia (χ2=4.471,P=0.034;χ2=4.206,P=0.040),rs1022528 locus was associated with illogic thought of paranoid type schizophrenia (χ2=4.108,P=0.043). Conclusion rs12026099 locus,rs2250312 locus and rs1022528 locus on PTGER3 gene are not associated with schizophrenia,but associated with clinical symptoms of schizophrenia in Han population from the north of China.

Key words: PTGER3 gene;schizophrenia /genetics;gene polymorphism;polymorphism,restriction fragment length