Abstract:

Abstract:Objective To study the influence of nitric oxide synthase(NOS) inhibitor L-aminoguanidine (L-AG) in the inflammatory factor levels in  degenerate intervertebral disc cells in vitro,and to explore its mechanism.Methods After treatment with L-AG(0,0.5%,1.0%,1.5%) for 24 h,the levels of NO and the activities of NOS were detected with spectrophotometric method and the expression levels of IL-1,IL-6 and TNF-α were determined by Western blotting.Results The activities of NOS in degenerate intervertebral disc cells in 0.5%,1.0%,1.5% L-AG groups were 6.1,5.5,and 4.2 U•mL^-1;the NO levels were 　 128.3,116.5, and 97.7  μmol•L-1,respectively,which were significantIy lower than those in OL-AG group(control group)（P<0.05）.Compared with control group，the expression levels of IL
^-1,IL-6 and TNF-α in  0.5%，1.0%，and 1.5%  L-AG  groups were also significantly reduced（P<0. 01-0.05）.Conclusion L-AG  can reduce the  inflammatory reaction of   degenerate intervertebral disc cells,which may be related to inhibition of NOS.

Key words: L-aminoguanidine, degenerate intervertebral disc cells, nitric oxide synthase, inflammatory factor