黄芩苷联合二甲双胍对多囊卵巢综合征大鼠内分泌、炎症因子和胰岛素抵抗的影响及其机制

doi:10.13481/j.1671-587X.20210311

Abstract

Abstract: Objective

To investigate the effects of baicalin （BAL）combined with metformin（DMBG） on the endocrine indicatros， inflammatory factors， endometrial condition and insulin resistance of the rats with polycystic ovary syndrome （PCOS） and their mechanisms.

Methods

A total of 32 rats were divided into control group， dehydroepiandrosterone（DHEA） group， DMBG group， and BAL + DMBG group，and there were 8 rats in each group.Except control group，the rats in other groups were given DHEA to establish the PCOS models.The blood of abdominal aorta of the rats was taken， and the levels of serum endocrine factorsluteinizing hormone（LH）， follicle-stimulating hormone（FSH）， testosterone（T），estradiol（E2）and tumor necrosis factor-α（TNF-α），C-reactive protein（CRP）， interleukin 6（IL-6）and interleukin 18（IL-18）were determined by enzyme-linked immunosorbent assay （ELISA）method； radioimmunoassay was used to detect the levels of fasting insulin（FINS）and fasting blood-glucose（FBG），and the insulin resistance index（HOMA-IR） was calculated；the area ratio of endometrial space to stroma and thickness of endometrium of the rats in various groups were detected；Western blotting method was used to detect the expression amounts of phosphorylated phosphatidylinositide 3-kinases （p-PI3K），phosphatidylinositide 3-kinases（PI3K）， phosphorylated protein kinase B （p-AKT） and protein kinase B （AKT） in ovary tissue of the rats in various groups.

Results

Compared with control group， the serum levels of FSH and E2 of the rats in DHEA group were decreased（P<0.05），and the serum levels of LH，T， TNF-α， CRP， IL-6，and IL-18 were increased （P<0.05），the serum levels of LH，T，FBG，FINS and HOMA-IR were increased （P<0.05），and the area ratio of endometrial space to stroma and the thickness of endometrium were decreased（P<0.05）.Compared with DHEA group， the serum levels of FSH， E2， TNF-α， CRP， IL-6，and IL-18 of the rats in BAL+DMBG group and DMBG group were increased （P<0.05），the serum levels of LH，T，FBG，FINS， and HOMA-IR were decreased （P<0.05），and the area ratios of endometrial space to stroma and the thicknesses of endometrium were increased（P<0.05）.Compared with DMBG group， the serum levels of FSH， E2， TNF-α， CRP， IL-6，and IL-18 of the rats in BAL+DMBG were increased （P<0.05），the serum levels of LH，T，FBG，FINS， and HOMA-IR were decreased （P<0.05）， and the area ratio of endometrial space to stroma and the thickness of endometrium were increased（P<0.05）.

Conclusion

BAL combined with DMBG can improve insulin resistance and endometrium condition by up-regulating the expressions of PI3K / AKT signaling pathway proteins in ovarian tissue of the PCOS rats，correcting endocrine disorders，and inhibiting the chronic low-grade inflammatory reaction.

Key words: baicalin, metformin, polycystic ovary syndrome, inflammatory factor, insulin resistance, endometrium condition

CLC Number:

R711.76

Lei XIE, Xiaoli LIU, Jing GE, Yawei LI. Effects of baicalin combined with metformin on endocrine, inflammatory factors and insulin resistance in rats with polycystic ovary syndrome and their mechanisms[J].Journal of Jilin University(Medicine Edition), 2021, 47(3): 623-629.

Figures/Tables 6

Tab. 1

Tab. 2

Tab. 3

Tab. 4

Fig. 1

Fig. 2

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Group	FSH [λ_B/(U·L^－1)]	E2[c_B/(pmol·L^－1)]	LH[λ_B/(U·L^－1)]	T [c_B/(mmol·L^－1)]
Control	7.66±1.05	46.90±5.63	2.65±0.36	1.78±0.25
DHEA	2.85±0.63^*	21.29±3.02^*	8.14±0.82^*	5.74±0.67^*
DMBG	5.10±0.78^△	33.71±2.10^△	5.45±1.56^△	3.79±0.31^△
BAL+DMBG	6.43±0.95^△#	39.30±2.14^△#	3.47±0.42^△#	2.68±0.32^△#

Group	TNF-α[ρ_B/(ng·L^－1)]	CRP [ρ_B/(mg·L^－1)]	IL-6[ρ_B/(ng·L^－1)]	IL-18[ρ_B/(μg·L^－1)]
Control	85.36±10.24	7.20±2.05	51.75±6.33	112.70±14.38
DHEA	177.78±20.60^*	15.67±3.63^*	99.63±14.85^*	197.29±23.69^*
DMBG	139.53±15.58^△	12.34±3.11^△	81.72±11.64^△	166.07±18.27^△
BAL+DMBG	101.71±12.43^△#	9.78±2.36^△#	66.50±8.69^△#	135.90±15.46^△#

Group	FBG [c_B/(mmol·L^－1)]	FINS [λ_B/(mIU·L^－1)]	HOMA-IR
Control	5.16±0.37	8.27±0.82	1.90±0.17
DHEA	6.29±0.45^*	14.62±1.49^*	4.09±0.51^*
DMBG	5.77±0.33^△	11.68±0.94^△	3.00±0.42^△
BAL+DMBG	5.39±0.31^△#	9.76±0.68^△#	2.34±0.25^△#

Group	Area ratio of endometrial space to stroma	Endometrium thickness (l/μm)
Control	0.50±0.19	67.95±5.73
DHEA	0.09±0.06^*	30.18±4.16^*
DMBG	0.21±0.10^△	41.23±4.78^△
BAL+DMBG	0.39±0.16^△#	55.61±5.22^△#

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Effects of baicalin combined with metformin on endocrine, inflammatory factors and insulin resistance in rats with polycystic ovary syndrome and their mechanisms

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