Abstract:

Abstract:Objective To investigate the effects of antidepressant olanzapine on the apoptosis,death  and cell cycle of  hippocampus neurons of the rats injured by glutamate (Glu),and to  illuminate the mechanism of neuroprotive effect of olanzapine.Methods The primary cultured hippocampal neurons were injured with Glu to set up Glu-injured models.The neurons  were divided into normal  control group,Glu injury group and olanzapine+Glu injury group.The changes of   apoptotic rate,death rate and cell cycle of neurons were detected by FCM,respectively.Results Compared with normal control group,the  apoptotic rate of neurons in the other  groups were increased (P<0.01);compared with Glu injury group,the apoptotic rate  of cells in 10.0 μmol/L olanzapine+Glu injury groupwas decreased(P<0.01).Compared with normal control group,the death rate of neurons was up to 2.9 times of that in Glu injury group(P<0.05);
compared with Glu injury group,the death rates of neurons in 100.0 and 200.0 μmol/L olanzapine+Glu groups were decreased (P<0.05 or P<0.01).Compored with normal control group,the  percentage of neurons in G0/G1 phase in Glu injury group was increased (P<0.01),and the  percentage of neurons in S phase was decreased (P<0.01),but the  percentage of neurons in G2+M phase didn’t change significantly;compared with Glu injury group,the
  percentages of   G0/G1 phase  in 50.0 and 100.0 μmol/L olanzapine+Glu injury groups were decreased to 79.9% and 62.6% of that in Glu injury group
(P<0.05 or P<0.01).and the  percentage of neurons in S phase  were up to 2.5 an 3.8 times of that in Glu injury group (P<0.05 or P<0.01).Conclusion
Olanzapine can prevent the apoptosis and death of hippocampal neurons induced by Glu,and change the cell cycle.