Abstract:

Abstract:Objective To observe the intervention effect of agaricus blazei polysaccharide (ABP) on ethology,mitochondrial membrane potential and apoptosis of hippocampal neuron in mice with brain aging induced by D-gal and to explore its effect and possible mechanism of improving the learning and memory ability of brain aging mice.Methods 40 Kunming mice were divided into four groups with 10 in each group:control group,aging model group,low dose ABP group and high dose ABP group.Morris water maze and avoiding dark instrument were used to detect the learning and memory ability of aging mice induced by D-gal while flow cytometry was used to analyze the changes of mitochondrial membrane potential and apoptosis.Results In the test of ethology,compared with model group,the percentages of distance in the central area of Morris water maze were obviously increased in ABP groups (P<0.05 or P<0.01),and the distances in the fourth quadrant were obviously decreased (P<0.01).Compared with model group,the mitochondrial membrane potential of hippocampal neuron in ABP groups were increased (P<0.01) and the apoptotic rates were decreased significantly (P<0.05 or P<0.01).Conclusion ABP can strengthen the learning and memory ability of aging mice and the mechanism is related to the increasing of  mitochondrial membrane potential of hippocampal neuron and the inhibition of apoptosis.

Key words: agaricus blazei polysaccharide, D-gal, brain aging, mitochondrial membrane potential, apoptosis