Abstract:

Objective To observe the synthesis of melanin  and tyrosinase activity of the cultured normal human melanocytes after treated with baicalin and NB-
UVB,and to provide theoretical  evidence for study on the  new therapy method of vitiligo.Methods The normal human menlanocytes cultured in vitro were
 divided into control group,different concentrations of baicalin groups,different doses of NB-UVB group and baicalin combined NB-UVB group.The proliferation rate of melanocytes was measured by MTT assay,the level  of melanin was tested by NaOH pyrolysis,and the tyrosinase activity was measured by dopa oxidization.Results Compared with control group,the proliferation rates in  10-4 mol/L baicalin group and 30 mJ/cm
  NB-UVB group had no significant difference（P>0.05）.Compared with control group,the levels  of melanin and tyrosinase activities in baicalin group,NB-
UVB group and combined group were  increased （P<0.05 or P<0.01）;compared with baicalin group and NB-UVB group,the level  of melanin
and tyrosinase activity  in combined group were increased（P<0.01）. Conclusion Baicalin combined with NB-UVB has better promotion effect on melanin synthesis than used alone.
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Key words:  , melanocytes, baicalin, narrwo band-UVB, melanin, tyrosinase