Based on the case-control study design， 34 cases of serum specimens from patients with definite histopathological diagnosis of gastric adenocarcinoma were selected as gastric cancer group， and 80 cases of serum specimens from healthy people who passed the physical examination were selected as control group. Luminex detection technology was used to detect the levels of gastric cancer-related cytokines in serum samples of the subjects in two groups. The tumor markers that were meaningful for the diagnosis of gastric cancer were screened from the following indexes such as macrophage inflammatory protein-3α （CCL20）， interleukin-2 （IL-2）， interleukin-4 （IL-4）， Interleukin-5 （IL-5）， interleukin-6 （IL-6）， interleukin-10 （IL-10）， interleukin-17A （IL-17A）， tumor necrosis factor-α （TNF-α）， interferon-γ （IFN-γ）， monocyte chemoattractant protein-4 （CCL13）， interferon-induced T cell alpha chemokine （CXCL11）， B-lymphocyte chemoattractant （CXCL13）， bone morphogenetic protein 4 （BMP4）， bone morphogenetic protein7 （BMP7）， hepatocyte growth factor （HGF）， granulocyte-macrophage colony stimulating factor （GM-CSF）， platelet derived growth factor-BB （PDGF-BB）.Quantitative real-time polymerase chain reaction（qRT-PCR） method was used to detect the serum miR-10b level of the patients in gastric cancer group；electrochemiluminescence method was used to detect the serum tumor markers carcinoembryonic antigen（CEA）， carbohydrate antigen 19-9（CA19-9） and carbohydrate antigen 72-4（CA72-4） levels of the patients in gastric cancer group.

Compared with control group， the serum levels of CCL20， IL-2， IL-4， IL-6， IL-17A， TNF-α， CCL13， BMP-4， and HGF of the patients in gastric cancer group were significantly increased （P<0.05）； the Logistic regression analysis results showed that the serum levels of CCL20， IL-2， IL-4， IL-6， IL-17A， TNF-α， CXCL11， CCL13， BMP-4， BMP-7， and HGF were associated with the risk of gastric cancer （ P<0.05）； the receptor operation characteristic curve（ROC） analysis results showed that the area under the curve （AUC） of CCL20 in diagnosis of gastric cancer was significantly better than combinnation diagnosis of the three tumor markers CEA， CA19-9，and CA72-4； When the cut-off value of CCL20 was 6.71 ng·L^－1， the diagnostic specificity， sensitivity， positive predictive value， negative predictive value， positive likelihood ratio， negative predictive ratio， positive likelihood ratio， negative predictive ratio and diagnostic accuracy were 76.5%， 91.3%， 78.8%， 90.1%， 8.74， 0.26，and 86.84%， respectively. The Spearman correlation analysis results showed that 17 kind of cytokines levels had poor correlations with miR-10b level， and the level of TNF-α had a certain correlation with miR-10b level（r=0.335， P=0.075）.

When 6.71 ng·L^－1 is used as the diagnostic cut-off value of the serum cytokine CCL20， its diagnostic sensitivity is significantly better than the tumor markers CEA， CA19-9 and CA72-4 commonly used in clinic， and the specificity is also higher.