化学和热传导双重损伤构建大鼠宫腔黏连模型的方法及其评价

doi:10.13481/j.1671-587X.20230129

Abstract

Abstract:

Objective To establish the rat models of intrauterine adhesion （IUA） with the chemical and heat conduction dual injury method， and to clarify its characteristics and advantages as an improved approach of chemical injury in constructing the animal model with similar pathological changes of IUA. Methods Six female SD rats aged 6－8 weeks were randomly divided into chemical injury group and chemical and heat conduction dual injury group （dual injury group），with 3 rats in each group.The left uterine horns of rats were used as experimental group and the right uterine horns were used as self-control group. The left uterine horns of the rats in chemical injury group， which were defined as the chemical injury experiment group， were treated with 95% ethanol for 3 min； the right uterine horns of the rats， which were defined as chemical injury self-control group，didn’t received any treatment. The left uterine horns of the rats in dual injury group， which were defined as dual injury experiment group， were treated with 95% ethanol in the left uterus for 3 min， and then treated with 100 ℃ hot water for 1 min； the right uterine horns of the rats， which were defined as dual injury self-control group， didn’t receive any treatment.On the 14 th day after modeling， the thickness of endometrial， the number of glands， the area of endometrial fibrosis and the proliferation index （PI） of glandular epithelial cells and stromal cells of the rats in various groups were detected by hematoxylin and eosin （HE） staining， Masson staining， and Ki67 immunohistochemical staining method. Results The HE staining results showed that compared with self-control group，the thickness of endometrium of the rats and the number of glands in chemical injury experiment group and dual injury experiment group were significantly reduced（P<0.05）.Compared with chemical injury experiment group， the uterine cavity of the rats in dual injury experiment group was closed， the endometrial thickness was reduced （P<0.05）， and the number of endometrial glands was decreased（P<0.05）. The Masson staining results showed that compared with self-control group， the fibrotic hyperplasia in endometrial layers of the rats in chemical injury experiment group and dual injury experiment group was seen， and the ratios of endometrial fibrosis area in exdometrium tissue were apparently increased （P<0.05）. Compared with chemical injury experiment group， fibrotic adhesion and intact myometrium of the rats in dual injury experiment group were found， and the ratio of endometrial fibrosis area was apparently increased （P<0.05）. The Ki67 immunohistochemical staining results showed that compared with self-control group， the nuclear shape and arrangement of glandular epithelial cells appeared the changes in endometrium tissue of the rats in chemical injury experiment group and dual injury experiment group， and the PI was significantly increased （P<0.05）. Conclusion Chemical and heat conduction dual injury method is an easy and reproducible operation that can establish the IUA model with fibrotic adhesion and intact muscle layer for the safety evaluation， mechanism research and clinical transformation of IUA， which possesses a good application perspect.

Key words: Intrauterine adhesion, Animal model, Endometrium, Chemical injury method, Heat conduction injury method

CLC Number:

R711.74

Lincui DA,Beihong ZHENG,Yan SUN,Suzhu CHEN,Yunhong LIN,Qingfen CHEN,Shengrong DU. Method to establish rat model of intrauterine adhesion through chemical and heat conduction dual injury and its evaluation[J].Journal of Jilin University(Medicine Edition), 2023, 49(1): 215-221.

Figures/Tables 6

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References 27

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Group	Thickness of endometrial（l/μm）	Number of glands
Chemical injury self-control	555.09±34.03	5.60±0.40
Chemical injury experiment	428.91±68.62^*	2.87±1.21^*
Dual injury self-control	518.70±36.46	4.67±1.03
Dual injury experiment	332.51±25.88^△#	1.80±0.53^△#

Group	PI
Group	Glandular epithelial cells	Stromal cells
Chemical injury self-control	428.64±135.98	244.60±110.21
Chemical injury experiment	601.10±164.16	549.42±67.23^*
Dual injury self-control	433.60±99.67	184.63±135.27
Dual injury experiment	568.97±99.04	579.93±158.54^△

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Method to establish rat model of intrauterine adhesion through chemical and heat conduction dual injury and its evaluation

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