外周血淋巴细胞亚群与颈动脉粥样硬化发生的关联性分析

doi:10.13481/j.1671-587X.20240525

Abstract

Abstract:

Objective： To analyze the association between the peripheral blood lymphocyte subsets and the occurrence of carotid atherosclerosis （CAS） and discuss the risk factors of CAS， and to provide the basis for risk prediction and early intervention of CAS. Methods A total of 419 subjects were selected according to the inclusion and exclusion criteria.All the subjects underwent carotid ultrasonography， and the detection of T lymphocyte， B lymphocyte， and natural killer（NK） cell subsets were performed. According to the carotid artery intima-media thickness（IMT）， the subjects were divided into normal carotid artery group （n=166） and CAS group （n=253）. The basic data and laboratory inspection indexes of the subjects in two groups were collected， including age， gender， blood pressure， medical history， smoking history， medication history， blood lipids， and percentages of T lymphocyte， B lymphocyte， and NK cell subsets. The risk factors for the occurrence of CAS of the subjects were analyzed using Logistic regression analysis. Results The univariate analysis results showed that compared with normal carotid artery group， the age， morbidity rate of hypertension， morbidity rate of diabetes， and smoking rate of the subjects in CAS group were increased （P<0.05）， and the percentages of CD4+ T lymphocytes， CD4+ T lymphocyte count， CD4+/CD8+ ratio， CD16+CD56+NK cell count， and low-density lipoprotein cholesterol （LDL-c） level in peripheral blood were increased （P<0.05）， while the percentage of CD8+T lymphocyte was decreased （P<0.05）. The multivariable Logistic regression analysis results showed that age （OR=1.112，95%CI：1.083－1.142， P<0.001）， smoking （OR=1.997，95%CI：1.192－3.346， P=0.009），LDL-c （OR=1.427， 95%CI：1.017－2.001， P=0.039）， and percentage of CD4+T lymphocyte（OR=1.044，95%CI：1.002－1.087，P=0.039） were the independent risk factors for the occurrence of CAS. Conclusion The percentage of CD4+ T lymphocyte is associated with the occurrence of CAS and it is a predictive risk factor for CAS.

Key words: Atherosclerosis, Carotid atherosclerosis, Risk factor, Natural killer cell, Low density lipoprotein cholesterol

CLC Number:

R543.4

Jiayuan YU,Di ZHAO,Xin JIANG,Jing XU,Lili JIANG,Hongyu JIANG. Association analysis on peripheral blood lymphocyte subsets and occurrence of carotid atherosclerosis[J].Journal of Jilin University(Medicine Edition), 2024, 50(5): 1400-1405.

Figures/Tables 4

Tab.1

Tab.2

Tab. 3

Tab.4

References 25

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Group	n	Male[n（η/%）]	Age（year）	Hypertension[n（η/%）]	Diabetes[n（η/%）]	Smoking[n（η/%）]
Normal carotid artery	166	92（55.4）	47.84±10.08	49（29.5）	25（15.1）	41（24.7）
CAS	253	152（60.1）	58.72±9.57	109（43.1）	75（29.6）	103（40.7）
χ²/t		0.894	－11.139	7.852	11.733	11.394
P		0.344	<0.001	0.005	0.001	0.001

Group	n	TC [c_B/（mmol·L^－1）]	TG [c_B/（mmol·L^－1）]		HDL-c [c_B/（mmol·L^－1）]		LDL-c [c_B/（mmol·L^－1）]
Normal carotid artery	166	5.25±0.95	1.45(1.04,2.27)		1.22(1.07,1.48)		3.20(2.76,3.54)
CAS	253	5.45±1.15	1.67(1.14,2.43)		1.24(1.09,1.44)		3.25(2.82,3.89)
Z/t		－1.893	－1.858		－0.012		－2.726
P		0.059	0.063		0.990		0.006
Group	n	CD3+T lymphocyte percentage（η/%）	CD3+T cell count （cells·μL^－1）		CD4+T lymphocyte percentage（η/%）		CD4+T cell count （cells·μL^－1）
Normal carotid artery	166	69.15(63.95,75.10)	1 178.00(1 012.75,1 542.00)		37.26±7.44		631.50(510.25,805.75)
CAS	253	67.80(61.50,73.65)	1 235.00(977.50,1 560.50)		39.11±7.68		738.00(546.50,924.00)
Z/t		－1.542	－0.602		－2.446		－2.281
P		0.123	0.547		0.015		0.023
Group	n	CD8+T lymphocyte percentage（η/%）	CD8+T cell count （cells·μL^－1）		CD4+/CD8+ratio		CD19+B lymphocyte percentage（η/%）
Normal carotid artery	166	25.10(20.75,31.10)	450.00(339.00,575.50)		1.43(1.15,1.90)		11.75(9.48,15.00)
CAS	253	23.60(18.55,28.85)	429.00(314.50,555.50)		1.67(1.27,2.33)		12.00(9.05,15.20)
Z/t		－2.786	－1.066		－3.430		－0.127
P		0.005	0.286		0.001		0.899
Group	n	CD19+B cell count （cells·μL^－1）		CD16+CD56+NK cell percentage（η/%）		CD16+CD56+NK cell count（cells·μL^－1）
Normal carotid artery	166	210.50(152.75,285.50)		16.20(12.00,21.65)		273.00(195.75,406.00)
CAS	253	211.00(161.00,304.50)		17.40(12.40,23.15)		314.00(220.00,451.50)
Z/t		－0.576		－1.229		－2.002
P		0.565		0.219		0.045

Variable	Assignment
Dependent variable	0 = Normal carotid artery group 1 = CAS group
Independent variable
Hypertension	0 = No；1 = Yes
Diabetes	0 = No；1 = Yes
Smoking	0 = No；1 = Yes
Age	Continuous variable
LDL-c	Continuous variable
CD4+T lymphocyte percentage	Continuous variable
CD8+T lymphocyte percentage	Continuous variable
CD16+CD56+NK cell count	Continuous variable

Risk factor	B	SE	χ²	OR	95%CI		P
Risk factor	B	SE	χ²	OR	Lower limit	Upper limit	P
Age	0.106	0.014	61.433	1.112	1.083	1.142	<0.001
LDL-c	0.355	0.173	4.240	1.427	1.017	2.001	0.039
Hypertension	0.073	0.257	0.081	1.076	0.650	1.782	0.775
Diabetes	0.313	0.297	1.107	1.367	0.763	2.449	0.293
Smoking	0.692	0.263	6.907	1.997	1.192	3.346	0.009
CD4+T lymphocyte percentage	0.043	0.021	4.274	1.044	1.002	1.087	0.039
CD8+T lymphocyte percentage	0.002	0.018	0.008	1.002	0.966	1.039	0.929
CD16+CD56+NK cell count	0.001	0.001	2.873	1.001	1.000	1.003	0.090

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Association analysis on peripheral blood lymphocyte subsets and occurrence of carotid atherosclerosis

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