利用核酸适配体修饰氧化石墨烯复合物的17β-雌二醇检测机理

doi:10.13278/j.cnki.jjuese.20190052

摘要/Abstract

摘要： 为了提高复杂环境水体中雌激素污染的检测性能，通过直接吸附法将核酸适配体固定在氧化石墨烯表面，合成出核酸适配体修饰氧化石墨烯复合物，并将其应用于17β-雌二醇水体污染的高灵敏和特异性均相检测。采用紫外可见分光光谱分析核酸适配体修饰氧化石墨烯复合物，发现该复合物在250 nm处有较宽的肩峰，证明核酸适配体成功固定在氧化石墨烯表面。通过荧光分光光度计分析核酸适配体修饰氧化石墨烯复合物与目标物17β-雌二醇反应前后的荧光强度变化，发现荧光强度由峰值110增加到峰值450，表明该复合物可以成功用于对17β-雌二醇的检测，并且荧光强度与17β-雌二醇的质量浓度在10~100 μg/L范围内呈正比，最低检出限为6.2 μg/L。

关键词: 氧化石墨烯, 核酸适配体, 17β-雌二醇, 荧光检测, 荧光共振能量转移

Abstract: In order to improve the detection performance of 17β-estradiol in complex environment water, the aptamer-modified graphene oxide complex was synthesized by immobilization of the aptamer on the surface of graphene oxide with direct adsorption,and it was used for high sensitive and specific homogeneous detection of 17β-estradiol contamination in water. The ultraviolet-visible spectroscopy was used to characterize the aptamer-modified graphene oxide complex. It was found that the complex had a wide peak at 250 nm, which proved that the nucleic acid aptamer was successfully immobilized on the surface of graphene oxide. The fluorescence intensity of the aptamer-modified graphene oxide complex before and after reaction with target 17β-estradiol was analyzed by the fluorescence spectrophotometer. It was found that the fluorescence intensities increased from 110 to 450, and the relative fluorescence intensities were proportional to the mass concentrations of 17β-estradiol in the range of 10-100 μg/L with a minimum detection limit of 6.2 μg/L. This indicates that the complex could be successfully used for the detection of 17β-estradiol.

Key words: graphene oxide, aptamer, 17β-estradiol, fluorescence detection, fluorescence resonance energy transfer

中图分类号:

P641

朴云仙, 胡慧, 姚兰, 张彧, 梁丽娜, 刘再冉. 利用核酸适配体修饰氧化石墨烯复合物的17β-雌二醇检测机理[J]. 吉林大学学报(地球科学版), 2020, 50(4): 1189-1196.

Piao Yunxian, Hu Hui, Yao Lan, Zhang Yu, Liang Lina, Liu Zairan. Bio-Sensing of 17β-Estradiol Using Aptamer Modified Graphene Oxide[J]. Journal of Jilin University(Earth Science Edition), 2020, 50(4): 1189-1196.

参考文献

[1] Novoselov K S, Geim A K, Morozov S V, et al. Electric Field Effect in Atomically Thin Carbon Films[J]. Science 2004, 306:666-669.
[2] Liu N, Liang G, Dong X, et. Stabilized Magnetic al Enzyme Aggregates on Graphene Oxide for High Performance Phenol and Bisphenol Removal[J]. Chemical Engineering Journal, 2016, 306:1026-1034.
[3] 陈晨,张祖培,卢文阁,等. 工程陶瓷及特种石墨在热熔器结构设计中的应用[J]. 吉林大学学报(地球科学版), 2004,34(4):643-647. Chen Chen, Zhang Zupei, Lu Wenge, et al.The Application of Engineering Ceramics and Special Graphite in Construction Design of Subterrene Drills[J]. Journal of Jilin University (Earth Science Edition), 2004, 34(4):643-647.
[4] Zeng Q O, Cheng J S, Tang L H, et al. Self-Assembled Graphene-Enzyme Hierarchical Nanostructures for Electrochemical Biosensing[J]. Advanced Functional Materials, 2010, 20(19):3366-3372.
[5] Wang Y, Li Z, Hu D, et al. Aptamer/Graphene Oxide Nano Complex for in Situ Molecular Probing in Living Cells[J]. Journal of the American Chemical Socoety, 2010, 132(27):9274-9276.
[6] Wang Y, Lu J, Tang L, et al. Graphene Oxide Amplified Electrogenerated Chemiluminescence of Quantum Dots and Its Selective Sensing for Glutathione from Thiol-Containing Compounds[J]. Analytical Chemistry, 2009, 81(23):9710-9715.
[7] 刘娜,梁刚,董新维,等. 酪氨酸酶固定化碳材料对苯酚的生物降解性能[J]. 吉林大学学报(地球科学版), 2017, 47(2):573-579. Liu Na, Liang Gang, Dong Xinwei, et al. Biodegradation Property of Phenol Using the Immobilized Tyrosinase on Carbon Material[J]. Journal of Jilin University (Earth Science Edition), 2017, 47(2):573-579.
[8] Yan H, Xu Y, Lu Y, et al. Reduced Graphene Oxide-Based Solid Phase Extraction for the Enrichment and Detection of MicroRNA[J]. Analytical Chemistry, 2017, 89(19):10137-10140.
[9] Wu M, Kempaiah R, Huang P J, et al. Adsorption and Desorption of DNA on Graphene Oxide Studied by Fluorescently Labeled Oligonucleotides[J]. Langmuir, 2011, 27(6):2731-2738.
[10] Liu M, Song J, Shuang S, et al. A Graphene-Based Biosensing Platform Based on the Release of DNA Probes and Rolling Circle Amplification[J]. ACS Nano, 2014, 8(6):5564-5573.
[11] Luo Y, Shi Z, Gao Q, et al. Magnetic Retrieval of Graphene:Extraction of Sulfonamide Antibiotics from Environmental Water Samples[J]. Journal of Chromatography A, 2011, 1218(10):1353-1358.
[12] Robertson D L, Joyce G F. Selection In Vitro of an RNA Enzyme that Specifically Cleaves Single-Stranded DNA[J]. Nature, 1990, 344:467-468.
[13] Tuerk C, Gold L. Systematic Evolution of Ligands by Exponential Enrichment:RNA Ligands to Bacteriophage T4 DNA Polymerase[J]. Science, 1990, 249:505-510.
[14] Ellington A D, Szostak J W. In Vitro Selectionof RNA Molecules that Bind Specific Ligands[J]. Nature, 1990, 346:818-822.
[15] Svobodova M, Skourodou V, Botero M, et al. The Characterizaion and Validation of 17β-Estradiol Binding Aptamers[J]. Journal of Steroid Biochemistry and Molecular Biology, 2017, 167:14-22.
[16] Taghdisi S M, Danesh N M, Ramezani M, et al. A Novel M-Shape Electrochemical Aptasensor for Ultrasensitive Detection of Tetracyclines[J]. Biosensor and Bioelectronic, 2016, 85:509-514.
[17] Gijs M, Penner G, Blackler G B, et al. Improved Aptamers for the Diagnosis and Potential Treatment of HER2-Positive Cancer[J]. Pharmaceuticals, 2016, 9(2), 1-21.
[18] 杨悦锁,张戈,宋晓明,等. 地下水和土壤环境中雌激素运移和归宿的研究进展[J]. 吉林大学学报(地球科学版), 2016, 46(4):1176-1190. Yang Yuesuo, Zhang Ge, Song Xiaoming, et al. Transport and Fate of Estrogens in Soil and Groundwater:A Critical Review[J]. Journal of Jilin University (Earth Science Edition), 2016, 46(4):1176-1190.
[19] Lucas S D, Jones D L. Biodegradation of Estrone and 17β-Estradiol in Grassland Soils Amended with Animal Wastes[J]. Soil Biology and Biochemistry, 2006, 38(9); 2803-2815.
[20] Dong X, He L, Liu Y, et al. Preparation of Highly Conductive Biochar Nanoparticles for Rapid and Sensitive Detection of 17β-Estradiol in Water[J]. Electrochimica Acta, 2018, 292:55-62.
[21] Draisci R, Purificato I, Delli Quadri F, et al. Development of an Electrochemical ELISA for the Screening of 17β-Estradiol and Application to Bovine Serum[J]. Analyst, 125(8):1419-1423.
[22] Hu L, Cheng Q, Chen D, et al.Liquid-Phase Exfoliated Graphene as Highly-Sensitive Sensor for Simultaneous Determination of Endocrine Disruptors:Diethylstilbestrol and Estradiol[J]. Journal of Hazardous Materials, 2015, 283:157-163.
[23] Fan L, Zhao G, Shi H, et al. A Femtomolar Level and Highly Selective 17β-Estradiol Photoelectrochemical Aptasensor Applied in Environmental Water Samples Analysis[J]. Environmental Science & Technology, 2014, 48(10):5754-5761.
[24] Amorim K P, Andrade L S. Development and Application of a Cloud Point Method for the Extraction of Natural Estrogens E1 and E2 from Urine Samples and Their Simultaneous Determination by HPLC-EC Using a BDD Electrode[J]. Analytical Methods, 2017, 9:1627-1633.
[25] 孙思明, 周焕英, 房彦军,等. 雌二醇的免疫胶体金试纸法检测[J]. 中国公共卫生, 2007, 23(1):126-127. Sun Siming, Zhou Huanying, Fang Yanjun, et al. Detection of Estradiol by Immune Colloidal-Gold Strips Method[J]. Chinese Journal of Public Health, 2007, 23(1):126-127.
[26] 张庆峰, 高志贤, 王升启. 用于雌二醇检测的免疫芯片技术[J]. 中国生物工程杂志, 2004, 24(9), 86-88. Zhang Qingfeng, Gao Zhixian, Wang Shengqi.Immunochip Techniques for Detection of 17β-Estradiol[J]. China Biotechnology, 2004, 24(9), 86-88.
[27] 朴云仙,祁小丽,胡慧,等. 基于核酸适配体功能化石墨纳米颗粒荧光探针的17β-雌二醇快速检测方法[J]. 吉林大学学报(地球科学版),2019,49(4):1137-1144. Piao Yunxian, Qi Xiaoli, Hu Hui, et al. A Method for Rapid Detection of 17β-Estradiol Based on Aptamer-Functionalized Graphite Nanoparticle as Fluorescent Probe[J]. Journal of Jilin University (Earth Science Edition),2019,49(4):1137-1144.
[28] Yildirim N, Long F, Gao C, et al. Aptamer-Based Optical Biosensor for Rapid and Sensitive Detection of 17β-Estradiol in Water Samples[J]. Environmental Science & Technology, 2012, 46(6):3288-3294.
[29] Huang K, Liu Y, Zhang J, et al. Aptamer/Au Nanoparticles/Cobalt Sulfide Nanosheets Biosensor for 17β-Estradiol Detection Using a Guanine-Rich Complementary DNA Sequence for Signal Amplification[J]. Biosensors and Bioelectronics, 2015, 67:184-191.
[30] Alsager O A, Kumar S,Zhu B, et al. Ultrasensitive Colorimetric Detection of 17β-Estradiol:The Effect of Shortening DNA Aptamer Sequences[J]. Analytical Chemistry, 2015, 87(8):4201-4209.
[31] Ai F, Zhong Y, Hu X, et al. Characterization on the Exfoliation Degree of Graphite Oxide into Graphene Oxide by UV-Visible Spectroscopy[J]. Journal of Wuhan University of Technology-Mater, 2016, 31(3):515-518.

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