假单胞菌DN2对多氯联苯的降解及bphA1核心序列测定

假单胞菌DN2对多氯联苯的降解及bphA1核心序列测定

任何军，刘娜，高松，张兰英，张玉玲，周睿

吉林大学环境与资源学院/吉林省水资源与水环境重点实验室,长春 130026

收稿日期:2008-05-08 修回日期:1900-01-01 出版日期:2009-03-26 发布日期:2009-03-26
通讯作者: 张兰英

Degradation of Polychlorinated Biphenyls and Confirm of bphA1 Gene Core by Pseudomonas DN2

REN He-jun, LIU Na, GAO Song,ZHANG Lan-ying，ZHANG Yu-ling，ZHOU Rui

College of Environment and Resources/Key Laboratory of WFEP, Jilin University， Changchun 130026，China

Received:2008-05-08 Revised:1900-01-01 Online:2009-03-26 Published:2009-03-26
Contact: ZHANG Lan-ying

摘要/Abstract

摘要： 从长期受PCBs污染的土壤中经富集培养，筛选分离到1株能以联苯为唯一碳源和能源生长的革兰氏阴性细菌DN2。16SrDNA序列分析初步鉴定为Pseudomonas sp，利用变压器油测定了该菌对各不同氯取代同系物的降解率。结果表明，DN2可以高效降解多氯联苯，其对10 mg/L的Aroclor 1242总量降解可达67%左右。进一步对该菌的bphA1基因核心序列克隆和测序分析表明，与Pseudomonas sp kks102菌株的同源性最高为92%，核苷酸序列转换成氨基酸序列（202AA）后，经ClauxW2比对发现11个保守位点，具有Glu-X3-4-Asp-X2-His-X4-5-His结构域存在。

关键词: PCBs, 生物降解, bphA1, 假单胞菌

Abstract: A polychlorinated biphenyls-degrading DN2，which can use biphenyl as the sole carbon source and energy, was isolated from the long-term PCBs-contaminated soil by enrichment in the soil first. According to 16S rDNA sequence analysis results，the strain was identified as Pseudomonas sp preliminarily. This pure strain was tested for biodegradation of several different PCBs by grew cells with transformer oil as food. The results showed the isolated strain degraded up to 67% of 10 mg/L Aroclor 1242. The bphA1 gene core, encoding the catalytic site of the large subunit of biphenyl dioxygenase, was also detected by PCR amplification and confirmed by DNA sequencing. It’s also showed Glu-X3-4-Asp-X2-His-X4-5-His domain existed in its constitution by bphA1 gene core.

Key words: PCBs, biodegradation, bphA1, Pseudomonas

中图分类号:

X172

任何军，刘娜，高松，张兰英，张玉玲，周睿. 假单胞菌DN2对多氯联苯的降解及bphA1核心序列测定[J]. J4, 2009, 39(2): 312-0316.

REN He-jun, LIU Na, GAO Song,ZHANG Lan-ying，ZHANG Yu-ling，ZHOU Rui. Degradation of Polychlorinated Biphenyls and Confirm of bphA1 Gene Core by Pseudomonas DN2[J]. J4, 2009, 39(2): 312-0316.

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